ID   DEF1_ECHCG              Reviewed;          47 AA.
AC   P86518;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   25-MAY-2022, entry version 18.
DE   RecName: Full=Defensin Ec-AMP-D1 {ECO:0000303|PubMed:18625284};
OS   Echinochloa crus-galli (Barnyard grass) (Panicum crus-galli).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Panicodae; Paniceae; Boivinellinae; Echinochloa.
OX   NCBI_TaxID=90397;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Seed {ECO:0000269|PubMed:18625284};
RX   PubMed=18625284; DOI=10.1016/j.biochi.2008.06.007;
RA   Odintsova T.I., Rogozhin E.A., Baranov Y., Musolyamov A.K., Yalpani N.,
RA   Egorov T.A., Grishin E.V.;
RT   "Seed defensins of barnyard grass Echinochloa crusgalli (L.) Beauv.";
RL   Biochimie 90:1667-1673(2008).
CC   -!- FUNCTION: Has antifungal activity. Inhibits spore germination in
CC       F.graminearum (IC(50)=15 ug/ml), F.oxysporum (IC(50)=102 ug/ml),
CC       F.verticillioides (IC(50)=8.5 ug/ml) and D.maydis (IC(50)=12.5 ug/ml),
CC       but not in C.graminicola, B.cinerea and H.sativum at concentrations
CC       below 30 ug/ml. Inhibits hyphal development in P.infestans (IC(50)=25.5
CC       ug/ml), but not release of zoospores. At concentrations above 100
CC       ug/ml, induces morphological changes such as lysis of hyphae and
CC       sporangia in P.infestans. {ECO:0000269|PubMed:18625284}.
CC   -!- MASS SPECTROMETRY: Mass=5098; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:18625284};
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000255}.
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DR   AlphaFoldDB; P86518; -.
DR   SMR; P86518; -.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   PRINTS; PR00288; PUROTHIONIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Defensin; Direct protein sequencing; Disulfide bond;
KW   Fungicide; Plant defense.
FT   CHAIN           1..47
FT                   /note="Defensin Ec-AMP-D1"
FT                   /id="PRO_0000394407"
FT   DISULFID        3..47
FT                   /evidence="ECO:0000250|UniProtKB:Q8GTM0"
FT   DISULFID        14..34
FT                   /evidence="ECO:0000250|UniProtKB:Q8GTM0"
FT   DISULFID        20..41
FT                   /evidence="ECO:0000250|UniProtKB:Q8GTM0"
FT   DISULFID        24..43
FT                   /evidence="ECO:0000250|UniProtKB:Q8GTM0"
SQ   SEQUENCE   47 AA;  5108 MW;  D78A70D4DD3F561E CRC64;
     RECQSQSHRY KGACVHDTNC ASVCQTEGFS GGKCVGFRGR CFCTKAC