DEF1_GALME
ID DEF1_GALME Reviewed; 72 AA.
AC P85213; Q6QR79;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 2.
DT 25-MAY-2022, entry version 31.
DE RecName: Full=Defensin;
DE AltName: Full=Galiomicin;
DE Flags: Precursor;
OS Galleria mellonella (Greater wax moth).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Pyraloidea;
OC Pyralidae; Galleriinae; Galleria.
OX NCBI_TaxID=7137;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC TISSUE=Larval fat body;
RX PubMed=14728668; DOI=10.1111/j.1365-2583.2004.00462.x;
RA Lee Y.S., Yun E.K., Jang W.S., Kim I., Lee J.H., Park S.Y., Ryu K.S.,
RA Seo S.J., Kim C.H., Lee I.H.;
RT "Purification, cDNA cloning and expression of an insect defensin from the
RT great wax moth, Galleria mellonella.";
RL Insect Mol. Biol. 13:65-72(2004).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 30-72, FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, INDUCTION, MASS SPECTROMETRY, AND DISULFIDE BONDS.
RC TISSUE=Larval hemolymph {ECO:0000269|PubMed:17194500};
RX PubMed=17194500; DOI=10.1016/j.peptides.2006.11.010;
RA Cytrynska M., Mak P., Zdybicka-Barabas A., Suder P., Jakubowicz T.;
RT "Purification and characterization of eight peptides from Galleria
RT mellonella immune hemolymph.";
RL Peptides 28:533-546(2007).
RN [3]
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND INDUCTION BY A.NIGER
RP ALPHA-1,3-GLUCAN.
RX PubMed=34443685; DOI=10.3390/molecules26165097;
RA Staczek S., Zdybicka-Barabas A., Wojda I., Wiater A., Mak P., Suder P.,
RA Skrzypiec K., Cytrynska M.;
RT "Fungal alpha-1,3-Glucan as a New Pathogen-Associated Molecular Pattern in
RT the Insect Model Host Galleria mellonella.";
RL Molecules 26:5097-5097(2021).
CC -!- FUNCTION: Has antifungal activity against A.niger (MIC=2.1 uM),
CC C.albicans (MIC=8.5 uM), C.fructus (MIC=8.5 uM), F.oxysporum (MIC=16.9
CC uM), P.pastoris (MIC=16.9 uM), P.tannophilus (MIC=8.5 uM), T.harzianum
CC (MIC=4.2 uM), and Z.marxianus (MIC=8.5 uM). Lacks antibacterial
CC activity against the Gram-positive bacteria L.monocytogenes, M.luteus,
CC S.aureus, and S.lutea, and the Gram-negative bacteria E.coli D31,
CC E.coli ATCC 25922, and S.typhimurium. {ECO:0000269|PubMed:17194500}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00710,
CC ECO:0000269|PubMed:14728668, ECO:0000269|PubMed:17194500,
CC ECO:0000269|PubMed:34443685}. Note=Secreted in the hemolymph.
CC {ECO:0000269|PubMed:14728668, ECO:0000269|PubMed:17194500,
CC ECO:0000269|PubMed:34443685}.
CC -!- TISSUE SPECIFICITY: Hemolymph and midgut. {ECO:0000269|PubMed:14728668,
CC ECO:0000269|PubMed:17194500}.
CC -!- DEVELOPMENTAL STAGE: Expressed in fat body of last instar larvae.
CC {ECO:0000269|PubMed:34443685}.
CC -!- INDUCTION: By bacterial infection (PubMed:17194500). Induced by A.niger
CC alpha-1,3-glucan (PubMed:34443685). {ECO:0000269|PubMed:17194500,
CC ECO:0000269|PubMed:34443685}.
CC -!- MASS SPECTROMETRY: Mass=4716.75; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:14728668};
CC -!- MASS SPECTROMETRY: Mass=4714.6; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:17194500};
CC -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 2
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR EMBL; AY528421; AAS19170.1; -; mRNA.
DR AlphaFoldDB; P85213; -.
DR SMR; P85213; -.
DR Proteomes; UP000504614; Unplaced.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IDA:UniProtKB.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR001542; Defensin_invertebrate/fungal.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE 1: Evidence at protein level;
KW Antimicrobial; Defensin; Direct protein sequencing; Disulfide bond;
KW Fungicide; Immunity; Innate immunity; Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000269|PubMed:14728668,
FT ECO:0000269|PubMed:17194500"
FT CHAIN 30..72
FT /note="Defensin"
FT /id="PRO_0000298768"
FT DISULFID 36..61
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT ECO:0000269|PubMed:17194500"
FT DISULFID 47..69
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT ECO:0000269|PubMed:17194500"
FT DISULFID 51..71
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT ECO:0000269|PubMed:17194500"
FT CONFLICT 35
FT /note="S -> R (in Ref. 1; AAS19170)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 72 AA; 7823 MW; 707D4A4D34034E85 CRC64;
MAKNFQSVLL LVCLSFLVIV SSPQNAVQAD TLIGSCVWGA TNYTSDCNAE CKRRGYKGGH
CGSFLNVNCW CE
