ID   DEF1_HOLDI              Reviewed;          43 AA.
AC   Q7M426;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Holotricin-1;
OS   Holotrichia diomphalia (Korean black chafer).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Coleoptera; Polyphaga; Scarabaeiformia;
OC   Scarabaeidae; Melolonthinae; Holotrichia.
OX   NCBI_TaxID=33394;
RN   [1]
RP   PROTEIN SEQUENCE, DISULFIDE BONDS, AND CHARACTERIZATION.
RX   PubMed=7550103; DOI=10.1248/bpb.18.457;
RA   Lee S.Y., Moon H.J., Kawabata S., Kurata S., Natori S., Lee B.L.;
RT   "A sapecin homologue of Holotrichia diomphalia: purification, sequencing
RT   and determination of disulfide pairs.";
RL   Biol. Pharm. Bull. 18:457-459(1995).
CC   -!- FUNCTION: Shows potent antibacterial activity against Gram-positive
CC       bacteria.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Hemolymph.
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 1
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR   PIR; JC2554; JC2554.
DR   AlphaFoldDB; Q7M426; -.
DR   SMR; Q7M426; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR017982; Defensin_insect.
DR   InterPro; IPR001542; Defensin_invertebrate/fungal.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   Pfam; PF01097; Defensin_2; 1.
DR   PRINTS; PR00271; DEFENSIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Immunity; Innate immunity; Secreted.
FT   CHAIN           1..43
FT                   /note="Holotricin-1"
FT                   /id="PRO_0000074472"
FT   DISULFID        3..34
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT                   ECO:0000269|PubMed:7550103"
FT   DISULFID        20..39
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT                   ECO:0000269|PubMed:7550103"
FT   DISULFID        24..41
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT                   ECO:0000269|PubMed:7550103"
SQ   SEQUENCE   43 AA;  4508 MW;  7D61717A85D863A3 CRC64;
     VTCDLLSLQI KGIAINDSAC AAHCLAMRRK GGSCKQGVCV CRN