ID   DEF1_HORVU              Reviewed;          47 AA.
AC   P20230;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Defensin-like protein 1;
DE   AltName: Full=Gamma-hordothionin;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Endosperm;
RX   PubMed=2176600; DOI=10.1111/j.1432-1033.1990.tb15649.x;
RA   Mendez E., Moreno A., Colilla F.J., Pelaez F., Limas G.G., Mendez R.,
RA   Soriano F., Salinas M., de Haro C.;
RT   "Primary structure and inhibition of protein synthesis in eukaryotic cell-
RT   free system of a novel thionin, gamma-hordothionin, from barley
RT   endosperm.";
RL   Eur. J. Biochem. 194:533-539(1990).
RN   [2]
RP   STRUCTURE BY NMR.
RX   PubMed=8380707; DOI=10.1021/bi00053a041;
RA   Bruix M., Jimenez M.A., Santoro J., Gonzalez C., Colilla F.J., Mendez E.,
RA   Rico M.;
RT   "Solution structure of gamma 1-H and gamma 1-P thionins from barley and
RT   wheat endosperm determined by 1H-NMR: a structural motif common to toxic
RT   arthropod proteins.";
RL   Biochemistry 32:715-724(1993).
CC   -!- FUNCTION: Inhibits protein translation in cell-free systems.
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC   -!- CAUTION: Was initially thought to be a thionin.
CC       {ECO:0000305|PubMed:2176600}.
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DR   PIR; S13849; S13849.
DR   PDB; 1GPT; NMR; -; A=1-47.
DR   PDBsum; 1GPT; -.
DR   AlphaFoldDB; P20230; -.
DR   SMR; P20230; -.
DR   EvolutionaryTrace; P20230; -.
DR   ExpressionAtlas; P20230; baseline and differential.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   PRINTS; PR00288; PUROTHIONIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Fungicide; Plant defense.
FT   CHAIN           1..47
FT                   /note="Defensin-like protein 1"
FT                   /id="PRO_0000074239"
FT   DISULFID        3..47
FT   DISULFID        14..34
FT   DISULFID        20..41
FT   DISULFID        24..43
FT   STRAND          2..6
FT                   /evidence="ECO:0007829|PDB:1GPT"
FT   HELIX           17..27
FT                   /evidence="ECO:0007829|PDB:1GPT"
FT   STRAND          30..35
FT                   /evidence="ECO:0007829|PDB:1GPT"
FT   TURN            36..39
FT                   /evidence="ECO:0007829|PDB:1GPT"
FT   STRAND          40..46
FT                   /evidence="ECO:0007829|PDB:1GPT"
SQ   SEQUENCE   47 AA;  5250 MW;  D51DA034B381AE16 CRC64;
     RICRRRSAGF KGPCVSNKNC AQVCMQEGWG GGNCDGPLRR CKCMRRC