DEF1_LACTC
ID DEF1_LACTC Reviewed; 733 AA.
AC C5DGU9;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 25-MAY-2022, entry version 48.
DE RecName: Full=RNA polymerase II degradation factor 1;
GN Name=DEF1; OrderedLocusNames=KLTH0D08470g;
OS Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284)
OS (Yeast) (Kluyveromyces thermotolerans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Lachancea.
OX NCBI_TaxID=559295;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 56472 / CBS 6340 / NRRL Y-8284;
RX PubMed=19525356; DOI=10.1101/gr.091546.109;
RG The Genolevures Consortium;
RA Souciet J.-L., Dujon B., Gaillardin C., Johnston M., Baret P.V.,
RA Cliften P., Sherman D.J., Weissenbach J., Westhof E., Wincker P., Jubin C.,
RA Poulain J., Barbe V., Segurens B., Artiguenave F., Anthouard V.,
RA Vacherie B., Val M.-E., Fulton R.S., Minx P., Wilson R., Durrens P.,
RA Jean G., Marck C., Martin T., Nikolski M., Rolland T., Seret M.-L.,
RA Casaregola S., Despons L., Fairhead C., Fischer G., Lafontaine I., Leh V.,
RA Lemaire M., de Montigny J., Neuveglise C., Thierry A., Blanc-Lenfle I.,
RA Bleykasten C., Diffels J., Fritsch E., Frangeul L., Goeffon A.,
RA Jauniaux N., Kachouri-Lafond R., Payen C., Potier S., Pribylova L.,
RA Ozanne C., Richard G.-F., Sacerdot C., Straub M.-L., Talla E.;
RT "Comparative genomics of protoploid Saccharomycetaceae.";
RL Genome Res. 19:1696-1709(2009).
CC -!- FUNCTION: RNA polymerase II degradation factor recruits the
CC ubiquitination machinery to the RNA polymerase II for
CC polyubiquitination, removal and degradation, when RAD26 fails to
CC efficiently displace stalled RNA polymerase II. Also involved in
CC telomere length regulation (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEF1 family. {ECO:0000305}.
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DR EMBL; CU928168; CAR22641.1; -; Genomic_DNA.
DR RefSeq; XP_002553079.1; XM_002553033.1.
DR AlphaFoldDB; C5DGU9; -.
DR SMR; C5DGU9; -.
DR STRING; 559295.C5DGU9; -.
DR EnsemblFungi; CAR22641; CAR22641; KLTH0D08470g.
DR GeneID; 8295315; -.
DR KEGG; lth:KLTH0D08470g; -.
DR eggNOG; ENOG502S359; Eukaryota.
DR HOGENOM; CLU_023119_0_0_1; -.
DR InParanoid; C5DGU9; -.
DR OMA; NPYYHQY; -.
DR OrthoDB; 1463481at2759; -.
DR Proteomes; UP000002036; Chromosome D.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0043130; F:ubiquitin binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd14368; CUE_DEF1_like; 1.
DR InterPro; IPR003892; CUE.
DR InterPro; IPR041803; DEF1_CUE.
DR PROSITE; PS51140; CUE; 1.
PE 3: Inferred from homology;
KW Chromosome; DNA damage; DNA repair; DNA-binding; Nucleus;
KW Reference proteome; Telomere; Ubl conjugation pathway.
FT CHAIN 1..733
FT /note="RNA polymerase II degradation factor 1"
FT /id="PRO_0000405669"
FT DOMAIN 24..66
FT /note="CUE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00468"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 61..343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 366..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 518..590
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 633..682
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..84
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 85..113
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 114..130
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 139..154
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..202
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..294
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 314..343
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 418..497
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 527..561
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 733 AA; 80081 MW; 6CE8FDB5078897F2 CRC64;
MSTQSRKSNS SKQPHSTHKK LDPELKFKLE TLTELFPDWT NDDLIDLVQE YEDLETIIDK
ITSGAATKWD EVKKPSKKER QREQQQQQQQ QQQAQLAAQQ ATQPSSQSHH NNHTHISPSH
DGDHSHSAQT SHNHHQSKSS KFSSRERDSS SRSHKKSSNN AAASGPNGSG NARRERASGA
SRVPATSAAS AASANASAVQ PDHLKTAVSA AKTASSTSWA AMASDKKAAK QSAQAKKAEE
QQQEQQSPQQ AQHESTAPSP QQEAEPQSQS QSKSQPQSDN KSAESVSSTS TPATEDLEKP
KKMTWAAIVK PKTKPSVKKS EPLEELEDLK REAAQISTEE PEAAEKVIEQ VIEQVEQTPE
EVVEQVEVTV VPEEVSQESE EASAQQEETA EPAAPVVPAE PEAASSEEKA APAQPEQGTY
SPVAQQQQPQ QQQQPQQQQQ AQQQQQQPTA YSEDKQSQAQ QAQSQQAQSF YQAQPQQYGS
QTPQQTPQTL QQQNAAAAAA AAQQQYYMYQ NQFPGYSYPG MFDSQSYPAG YGQQYAPQSQ
NGSQPQTAST QQSQSGQYGV PPGYASTGRD LGAASPMAAQ VQLQQQQQQQ PYGGSFMPYY
HFYQQSFPYG QPQYGMAGQY PYQVPKAYNY MNQYQPQQGG QTPSSQSQQG EEAQQSAQQG
QATASQGQAQ GSGSQGQAQT NAQQQAQLQQ YYQFQQQQQQ QQAAAAAAAA QQGGVPYGYS
GYDFSSQATR GFY