ID   DEF1_MACMU              Reviewed;          96 AA.
AC   P60030; P82318;
DT   21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   21-NOV-2003, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Neutrophil defensin 1;
DE   AltName: Full=RMAD-1;
DE   Flags: Precursor;
OS   Macaca mulatta (Rhesus macaque).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9544;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 67-96, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Bone marrow, and Leukocyte;
RX   PubMed=10531277; DOI=10.1128/iai.67.11.6139-6144.1999;
RA   Tang Y.Q., Yuan J., Miller C.J., Selsted M.E.;
RT   "Isolation, characterization, cDNA cloning, and antimicrobial properties of
RT   two distinct subfamilies of alpha-defensins from rhesus macaque
RT   leukocytes.";
RL   Infect. Immun. 67:6139-6144(1999).
CC   -!- FUNCTION: Has bacteriostatic activity against Gram-positive bacteria
CC       S.aureus and L.monocytogenes and Gram-negative bacterium E.coli and
CC       antifungal activity against C.neoformans. Has microbicidial activity
CC       against Gram-positive bacteria S.aureus and L.monocytogenes.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MASS SPECTROMETRY: Mass=3446.9; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:10531277};
CC   -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
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DR   EMBL; AF188268; AAF06312.1; -; mRNA.
DR   PIR; A59076; A59076.
DR   RefSeq; XP_015000177.1; XM_015144691.1.
DR   AlphaFoldDB; P60030; -.
DR   STRING; 9544.ENSMMUP00000024362; -.
DR   PRIDE; P60030; -.
DR   Ensembl; ENSMMUT00000026035; ENSMMUP00000024362; ENSMMUG00000018517.
DR   GeneID; 574196; -.
DR   CTD; 728358; -.
DR   VEuPathDB; HostDB:ENSMMUG00000018517; -.
DR   eggNOG; ENOG502T2EX; Eukaryota.
DR   GeneTree; ENSGT00940000153268; -.
DR   HOGENOM; CLU_160803_0_0_1; -.
DR   InParanoid; P60030; -.
DR   OMA; ISFAWDE; -.
DR   TreeFam; TF338414; -.
DR   Proteomes; UP000006718; Chromosome 8.
DR   Bgee; ENSMMUG00000018517; Expressed in spleen and 15 other tissues.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR   GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IBA:GO_Central.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IBA:GO_Central.
DR   GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   GO; GO:0051673; P:membrane disruption in another organism; IBA:GO_Central.
DR   InterPro; IPR016327; Alpha-defensin.
DR   InterPro; IPR006081; Alpha-defensin_C.
DR   InterPro; IPR002366; Alpha-defensin_propep.
DR   InterPro; IPR006080; Defensin_beta/alpha.
DR   PANTHER; PTHR11876; PTHR11876; 1.
DR   Pfam; PF00323; Defensin_1; 1.
DR   Pfam; PF00879; Defensin_propep; 1.
DR   PIRSF; PIRSF001875; Alpha-defensin; 1.
DR   SMART; SM00048; DEFSN; 1.
DR   PROSITE; PS00269; DEFENSIN; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Phosphoprotein; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..66
FT                   /evidence="ECO:0000269|PubMed:10531277"
FT                   /id="PRO_0000006795"
FT   PEPTIDE         67..96
FT                   /note="Neutrophil defensin 1"
FT                   /id="PRO_0000006796"
FT   MOD_RES         87
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P59665"
FT   DISULFID        68..96
FT                   /evidence="ECO:0000250"
FT   DISULFID        70..85
FT                   /evidence="ECO:0000250"
FT   DISULFID        75..95
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   96 AA;  10544 MW;  BBC8A81AD8E304C5 CRC64;
     MRTLVILAAI LLVALQAQAE PLQARTDEAT AAQEQIPTDN PEVVVSLAWD ESLAPKDSVP
     GLRKNMACYC RIPACLAGER RYGTCFYLGR VWAFCC