ID   DEF1_NICPA              Reviewed;         106 AA.
AC   O24115;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Defensin-like protein 1;
DE   AltName: Full=Gamma-thionin 1;
DE   Flags: Precursor;
GN   Name=THIO1;
OS   Nicotiana paniculata.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=62141;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RA   Komori T., Yamada S., Imaseki H.;
RT   "A cDNA clone for gamma-thionin from Nicotiana paniculata.";
RL   (er) Plant Gene Register PGR97-132(1997).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Vacuole {ECO:0000250}.
CC   -!- INDUCTION: By salt stress.
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC   -!- CAUTION: Was initially thought (Ref.1) to be a thionin. {ECO:0000305}.
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DR   EMBL; AB005250; BAA21325.1; -; mRNA.
DR   AlphaFoldDB; O24115; -.
DR   SMR; O24115; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   PRINTS; PR00288; PUROTHIONIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   2: Evidence at transcript level;
KW   Antimicrobial; Disulfide bond; Fungicide; Plant defense; Secreted; Signal;
KW   Stress response; Vacuole.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..106
FT                   /note="Defensin-like protein 1"
FT                   /id="PRO_0000007044"
FT   DISULFID        29..73
FT                   /evidence="ECO:0000250"
FT   DISULFID        40..60
FT                   /evidence="ECO:0000250"
FT   DISULFID        46..67
FT                   /evidence="ECO:0000250"
FT   DISULFID        50..69
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   106 AA;  11722 MW;  269850E116D775A4 CRC64;
     MARSLCFMAF AVLAMMLFVA YEVQAKSTCK AESNTFPGLC ITKPPCRKAC LSEKFTDGKC
     SKILRRCICY KPCVFDGKMI QTGAENLAEE AETLAAALLE EEMMDN