ID   DEF1_SORBI              Reviewed;          47 AA.
AC   P21923;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Defensin-like protein 1;
DE   AltName: Full=Small protein inhibitor of insect alpha-amylases 1;
DE            Short=SI alpha-1;
OS   Sorghum bicolor (Sorghum) (Sorghum vulgare).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum.
OX   NCBI_TaxID=4558;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. French red; TISSUE=Seed;
RX   PubMed=1995329; DOI=10.1016/0014-5793(91)80261-z;
RA   Bloch C. Jr., Richardson M.;
RT   "A new family of small (5 kDa) protein inhibitors of insect alpha-amylases
RT   from seeds or sorghum (Sorghum bicolar (L) Moench) have sequence homologies
RT   with wheat gamma-purothionins.";
RL   FEBS Lett. 279:101-104(1991).
RN   [2]
RP   SEQUENCE REVISION TO 35.
RC   TISSUE=Seed;
RX   PubMed=7705336; DOI=10.1111/j.1432-1033.1995.tb20257.x;
RA   Nitti G., Orru S., Bloch C. Jr., Morhy L., Marino G., Pucci P.;
RT   "Amino acid sequence and disulphide-bridge pattern of three gamma-thionins
RT   from Sorghum bicolor.";
RL   Eur. J. Biochem. 228:250-256(1995).
RN   [3]
RP   STRUCTURE BY NMR.
RX   PubMed=9715910;
RX   DOI=10.1002/(sici)1097-0134(19980815)32:3<334::aid-prot9>3.0.co;2-h;
RA   Bloch C. Jr., Patel S.U., Baud F., Zvelebil M.J., Carr M.D., Sadler P.J.,
RA   Thornton J.M.;
RT   "1H NMR structure of an antifungal gamma-thionin protein SIalpha1:
RT   similarity to scorpion toxins.";
RL   Proteins 32:334-349(1998).
CC   -!- SIMILARITY: Belongs to the DEFL family. Protease inhibitor I18
CC       (RTI/MTI-2) subfamily. {ECO:0000305}.
CC   -!- CAUTION: Was initially thought (PubMed:1995329, PubMed:7705336 and
CC       PubMed:9715910) to be a protease inhibitor. {ECO:0000305}.
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DR   PIR; S69145; S69145.
DR   AlphaFoldDB; P21923; -.
DR   SMR; P21923; -.
DR   EnsemblPlants; OQU80070; OQU80070; SORBI_3007G075250.
DR   Gramene; OQU80070; OQU80070; SORBI_3007G075250.
DR   eggNOG; ENOG502R3RE; Eukaryota.
DR   ExpressionAtlas; P21923; baseline.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW   Plant defense.
FT   CHAIN           1..47
FT                   /note="Defensin-like protein 1"
FT                   /id="PRO_0000074248"
FT   DISULFID        3..47
FT   DISULFID        14..36
FT   DISULFID        20..41
FT   DISULFID        24..43
SQ   SEQUENCE   47 AA;  5382 MW;  4F0810A702232A0E CRC64;
     RVCMGKSQHH SFPCISDRLC SNECVKEEGG WTAGYCHLRY CRCQKAC