DEF1_WHEAT
ID DEF1_WHEAT Reviewed; 47 AA.
AC P20158;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Defensin-like protein 1;
DE AltName: Full=Gamma-1-purothionin;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP PROTEIN SEQUENCE.
RC STRAIN=cv. Senatore Capelli; TISSUE=Endosperm;
RX PubMed=2226781; DOI=10.1016/0014-5793(90)81265-p;
RA Colilla F.J., Rocher A., Mendez E.;
RT "Gamma-purothionins: amino acid sequence of two polypeptides of a new
RT family of thionins from wheat endosperm.";
RL FEBS Lett. 270:191-194(1990).
RN [2]
RP STRUCTURE BY NMR.
RX PubMed=8380707; DOI=10.1021/bi00053a041;
RA Bruix M., Jimenez M.A., Santoro J., Gonzalez C., Colilla F.J., Mendez E.,
RA Rico M.;
RT "Solution structure of gamma 1-H and gamma 1-P thionins from barley and
RT wheat endosperm determined by 1H-NMR: a structural motif common to toxic
RT arthropod proteins.";
RL Biochemistry 32:715-724(1993).
CC -!- FUNCTION: Inhibits protein translation in cell-free systems.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC -!- CAUTION: Was initially thought (PubMed:2226781 and PubMed:8380707) to
CC be a thionin. {ECO:0000305}.
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DR PDB; 1GPS; NMR; -; A=1-47.
DR PDBsum; 1GPS; -.
DR AlphaFoldDB; P20158; -.
DR SMR; P20158; -.
DR STRING; 4565.Traes_5BL_C4B27C0A9.1; -.
DR EnsemblPlants; TraesCAD_scaffold_000911_01G000100.1; TraesCAD_scaffold_000911_01G000100.1; TraesCAD_scaffold_000911_01G000100.
DR EnsemblPlants; TraesPAR_scaffold_002991_01G000300.1; TraesPAR_scaffold_002991_01G000300.1; TraesPAR_scaffold_002991_01G000300.
DR EnsemblPlants; TraesWEE_scaffold_003058_01G000400.1; TraesWEE_scaffold_003058_01G000400.1; TraesWEE_scaffold_003058_01G000400.
DR Gramene; TraesCAD_scaffold_000911_01G000100.1; TraesCAD_scaffold_000911_01G000100.1; TraesCAD_scaffold_000911_01G000100.
DR Gramene; TraesPAR_scaffold_002991_01G000300.1; TraesPAR_scaffold_002991_01G000300.1; TraesPAR_scaffold_002991_01G000300.
DR Gramene; TraesWEE_scaffold_003058_01G000400.1; TraesWEE_scaffold_003058_01G000400.1; TraesWEE_scaffold_003058_01G000400.
DR eggNOG; ENOG502S7BC; Eukaryota.
DR EvolutionaryTrace; P20158; -.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; P20158; baseline.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR PRINTS; PR00288; PUROTHIONIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW Fungicide; Plant defense; Reference proteome.
FT CHAIN 1..47
FT /note="Defensin-like protein 1"
FT /id="PRO_0000074261"
FT SITE 11
FT /note="Interaction with trypsin"
FT /evidence="ECO:0000250"
FT DISULFID 3..47
FT DISULFID 14..34
FT DISULFID 20..41
FT DISULFID 24..43
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:1GPS"
FT HELIX 17..26
FT /evidence="ECO:0007829|PDB:1GPS"
FT STRAND 30..35
FT /evidence="ECO:0007829|PDB:1GPS"
FT TURN 36..39
FT /evidence="ECO:0007829|PDB:1GPS"
FT STRAND 40..46
FT /evidence="ECO:0007829|PDB:1GPS"
SQ SEQUENCE 47 AA; 5239 MW; 8ACB73627381A4D8 CRC64;
KICRRRSAGF KGPCMSNKNC AQVCQQEGWG GGNCDGPFRR CKCIRQC
