ID   DEF2_MESMA              Reviewed;          61 AA.
AC   P0DQT8;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Defensin BmKDfsin2 {ECO:0000303|PubMed:24129506};
DE   Flags: Precursor;
OS   Mesobuthus martensii (Manchurian scorpion) (Buthus martensii).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus.
OX   NCBI_TaxID=34649;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   TISSUE=Muscle;
RX   PubMed=24129506; DOI=10.1038/ncomms3602;
RA   Cao Z., Yu Y., Wu Y., Hao P., Di Z., He Y., Chen Z., Yang W., Shen Z.,
RA   He X., Sheng J., Xu X., Pan B., Feng J., Yang X., Hong W., Zhao W., Li Z.,
RA   Huang K., Li T., Kong Y., Liu H., Jiang D., Zhang B., Hu J., Hu Y.,
RA   Wang B., Dai J., Yuan B., Feng Y., Huang W., Xing X., Zhao G., Li X.,
RA   Li Y., Li W.;
RT   "The genome of Mesobuthus martensii reveals a unique adaptation model of
RT   arthropods.";
RL   Nat. Commun. 4:2602-2602(2013).
CC   -!- FUNCTION: Antibacterial peptide active against Gram-positive bacteria,
CC       but not on Gram-negative bacteria (By similarity). Has also weak
CC       blocking activity on Kv1.1/KCNA1, Kv1.2/KCNA2, Kv1.3/KCNA3,
CC       KCa3.1/KCNN4/IK, KCa2.3/KCNN3/SK3 and Kv11.1/KCNH2/ERG1 channels
CC       (tested at 1 uM) (By similarity). It inhibits potassium channel current
CC       by interacting with the pore region (By similarity).
CC       {ECO:0000250|UniProtKB:A0A384E0Y8, ECO:0000250|UniProtKB:P0DQU0}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:24129506}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in non-venom gland (hemolymph) and
CC       moderately expressed in venom gland. {ECO:0000250|UniProtKB:P0DQU0}.
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 2
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR001542; Defensin_invertebrate/fungal.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   Pfam; PF01097; Defensin_2; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE   3: Inferred from homology;
KW   Antibiotic; Antimicrobial;
KW   Calcium-activated potassium channel impairing toxin; Defensin;
KW   Disulfide bond; Immunity; Innate immunity; Ion channel impairing toxin;
KW   Potassium channel impairing toxin; Secreted; Signal; Toxin;
KW   Voltage-gated potassium channel impairing toxin.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..61
FT                   /note="Defensin BmKDfsin2"
FT                   /evidence="ECO:0000305|PubMed:24129506"
FT                   /id="PRO_0000455526"
FT   DISULFID        28..49
FT                   /evidence="ECO:0000250|UniProtKB:A0A384E0Y8"
FT   DISULFID        35..57
FT                   /evidence="ECO:0000250|UniProtKB:A0A384E0Y8"
FT   DISULFID        39..59
FT                   /evidence="ECO:0000250|UniProtKB:A0A384E0Y8"
SQ   SEQUENCE   61 AA;  6726 MW;  FEE28E5289BBE12D CRC64;
     METIVLLFLL ALVFCTLEMG MVEAEHGCPD NEDECHEHCK SIGKSGGYCV GPHKQTCRCN
     P