DEF2_RAPSA
ID DEF2_RAPSA Reviewed; 80 AA.
AC P30230; Q003I2;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 4.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Defensin-like protein 2;
DE AltName: Full=Cysteine-rich antifungal protein 2;
DE Short=AFP2;
DE Short=RAFP2;
DE Flags: Precursor;
GN Name=AFP2;
OS Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus.
OX NCBI_TaxID=3726;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ronde Rode Kleine Witpunt; TISSUE=Seed;
RX PubMed=7780308; DOI=10.2307/3870116;
RA Terras F.R.G., Eggermont K., Kovaleva V., Raikhel N.V., Osborn R.W.,
RA Kester A., Rees S.B., Torrekens S., van Leuven F., Vanderleyden J.,
RA Cammue B.P.A., Broekaert W.F.;
RT "Small cysteine-rich antifungal proteins from radish: their role in host
RT defense.";
RL Plant Cell 7:573-588(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kulkarni A.V., Krishnaraj P.U., Kuruvinashetti M.S.;
RT "Cloning and expression of rafp2 antifungal gene.";
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 30-65.
RC TISSUE=Seed;
RX PubMed=1639777; DOI=10.1016/s0021-9258(19)49534-3;
RA Terras F.R.G., Schoofs H.M.E., de Bolle M.F.C., van Leuven F., Rees S.B.,
RA Vanderleyden J., Cammue B.P.A., Broekaert W.F.;
RT "Analysis of two novel classes of plant antifungal proteins from radish
RT (Raphanus sativus L.) seeds.";
RL J. Biol. Chem. 267:15301-15309(1992).
RN [4]
RP PROTEIN SEQUENCE OF 30-58, AND PYROGLUTAMATE FORMATION AT GLN-30.
RX PubMed=8422949; DOI=10.1016/0014-5793(93)81299-f;
RA Terras F.R.G., Torrekens S., van Leuven F., Osborn R.W., Vanderleyden J.,
RA Cammue B.P.A., Broekaert W.F.;
RT "A new family of basic cysteine-rich plant antifungal proteins from
RT Brassicaceae species.";
RL FEBS Lett. 316:233-240(1993).
RN [5]
RP FUNCTION.
RX PubMed=8663029; DOI=10.1074/jbc.271.25.15018;
RA Thevissen K., Ghazi A., De Samblanx G.W., Brownlee C., Osborn R.W.,
RA Broekaert W.F.;
RT "Fungal membrane responses induced by plant defensins and thionins.";
RL J. Biol. Chem. 271:15018-15025(1996).
RN [6]
RP MUTAGENESIS OF GLN-34; PRO-36; GLY-38; THR-39; SER-41; GLY-45; ILE-55;
RP LEU-57; ALA-60; ASN-66; TYR-67; VAL-68; PHE-69; PRO-70; ALA-71; LYS-73;
RP ILE-75; TYR-77 AND PHE-78.
RX PubMed=8995418; DOI=10.1074/jbc.272.2.1171;
RA De Samblanx G.W., Goderis I.J., Thevissen K., Raemaekers R., Fant F.,
RA Borremans F., Acland D.P., Osborn R.W., Patel S., Broekaert W.F.;
RT "Mutational analysis of a plant defensin from radish (Raphanus sativus L.)
RT reveals two adjacent sites important for antifungal activity.";
RL J. Biol. Chem. 272:1171-1179(1997).
CC -!- FUNCTION: Possesses antifungal activity sensitive to inorganic cations.
CC Induces potential changes in fungal membranes and increased K(+) efflux
CC and Ca(2+) uptake. {ECO:0000269|PubMed:8663029}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR EMBL; U18556; AAA69540.1; -; mRNA.
DR EMBL; DQ979838; ABJ09664.1; -; Genomic_DNA.
DR PIR; T10823; T10823.
DR PDB; 2N2R; NMR; -; A=30-80.
DR PDBsum; 2N2R; -.
DR AlphaFoldDB; P30230; -.
DR SMR; P30230; -.
DR Proteomes; UP000504610; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW Fungicide; Plant defense; Pyrrolidone carboxylic acid; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000269|PubMed:1639777"
FT CHAIN 30..80
FT /note="Defensin-like protein 2"
FT /id="PRO_0000007052"
FT MOD_RES 30
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:8422949"
FT DISULFID 33..80
FT /evidence="ECO:0000250"
FT DISULFID 44..65
FT /evidence="ECO:0000250"
FT DISULFID 50..74
FT /evidence="ECO:0000250"
FT DISULFID 54..76
FT /evidence="ECO:0000250"
FT MUTAGEN 34
FT /note="Q->M: Increased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 36
FT /note="P->R: No effect on antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 38
FT /note="G->R: Strongly increased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 39
FT /note="T->G: Decreased antifungal potency and increased
FT sensitivity to inorganic cations."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 41
FT /note="S->R: Slightly decreased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 45
FT /note="G->M: Increased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 55
FT /note="I->R: Slightly decreased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 57
FT /note="L->R: Decreased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 60
FT /note="A->W: Decreased antifungal potency and increased
FT sensitivity to inorganic cations."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 66
FT /note="N->R: No effect on antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 67
FT /note="Y->A: Decreased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 67
FT /note="Y->G: Decreased antifungal potency and increased
FT sensitivity to inorganic cations."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 68
FT /note="V->R: Strongly increased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 69
FT /note="F->M: Decreased antifungal potency and increased
FT sensitivity to inorganic cations."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 70
FT /note="Missing: Decreased antifungal potency and increased
FT sensitivity to inorganic cations."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 71
FT /note="A->R: Slightly decreased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 73
FT /note="K->Q: Decreased antifungal potency and increased
FT sensitivity to inorganic cations."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 75
FT /note="I->R: Decreased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 77
FT /note="Y->I: No effect on antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT MUTAGEN 78
FT /note="F->R: Slightly decreased antifungal potency."
FT /evidence="ECO:0000269|PubMed:8995418"
FT STRAND 32..35
FT /evidence="ECO:0007829|PDB:2N2R"
FT HELIX 47..57
FT /evidence="ECO:0007829|PDB:2N2R"
FT STRAND 61..68
FT /evidence="ECO:0007829|PDB:2N2R"
FT STRAND 71..79
FT /evidence="ECO:0007829|PDB:2N2R"
SQ SEQUENCE 80 AA; 8875 MW; 746110D9A8CE6370 CRC64;
MAKFASIIVL LFVALVVFAA FEEPTMVEAQ KLCQRPSGTW SGVCGNNNAC KNQCIRLEKA
RHGSCNYVFP AHKCICYFPC
