ID   DEF2_RAT                Reviewed;          94 AA.
AC   Q62715;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Neutrophil antibiotic peptide NP-2;
DE            Short=RatNP-2;
DE   AltName: Full=Neutrophil defensin 2;
DE   Flags: Precursor;
GN   Name=Defa;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Bone marrow;
RX   PubMed=7594610;
RA   Yount N.Y., Wang M.-S.C., Yuan J., Banaiee N., Ouellette A.J.,
RA   Selsted M.E.;
RT   "Rat neutrophil defensins. Precursor structures and expression during
RT   neutrophilic myelopoiesis.";
RL   J. Immunol. 155:4476-4484(1995).
RN   [2]
RP   PROTEIN SEQUENCE OF 63-94.
RC   STRAIN=Sprague-Dawley; TISSUE=Neutrophil;
RX   PubMed=2254017; DOI=10.1128/iai.58.12.3899-3902.1990;
RA   Eisenhauer P.B., Harwig S.S.S.L., Szklarek D., Ganz T., Lehrer R.I.;
RT   "Polymorphic expression of defensins in neutrophils from outbred rats.";
RL   Infect. Immun. 58:3899-3902(1990).
CC   -!- FUNCTION: Active in vitro against S.aureus, fungi, Gram-positive and
CC       Gram-negative bacteria and to a lesser extent against an enveloped
CC       virus.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Highest expression in bone marrow and to a much
CC       lesser extent in small intestine.
CC   -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
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DR   EMBL; U16685; AAA91973.1; -; mRNA.
DR   PIR; E61014; E61014.
DR   RefSeq; NP_775451.2; NM_173329.2.
DR   AlphaFoldDB; Q62715; -.
DR   SMR; Q62715; -.
DR   STRING; 10116.ENSRNOP00000018501; -.
DR   PaxDb; Q62715; -.
DR   PRIDE; Q62715; -.
DR   Ensembl; ENSRNOT00000018501; ENSRNOP00000018501; ENSRNOG00000030093.
DR   GeneID; 286995; -.
DR   KEGG; rno:286995; -.
DR   UCSC; RGD:727951; rat.
DR   CTD; 1670; -.
DR   RGD; 727951; Defa.
DR   eggNOG; ENOG502T2EX; Eukaryota.
DR   GeneTree; ENSGT00940000153268; -.
DR   HOGENOM; CLU_160803_0_0_1; -.
DR   InParanoid; Q62715; -.
DR   OMA; ESHTGRC; -.
DR   OrthoDB; 1610714at2759; -.
DR   PhylomeDB; Q62715; -.
DR   TreeFam; TF338414; -.
DR   Reactome; R-RNO-1461973; Defensins.
DR   Reactome; R-RNO-1462054; Alpha-defensins.
DR   Reactome; R-RNO-6798695; Neutrophil degranulation.
DR   PRO; PR:Q62715; -.
DR   Proteomes; UP000002494; Chromosome 16.
DR   Bgee; ENSRNOG00000030093; Expressed in thymus and 8 other tissues.
DR   Genevisible; Q62715; RN.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR   GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR   GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR   GO; GO:0042742; P:defense response to bacterium; TAS:RGD.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; ISO:RGD.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; ISO:RGD.
DR   GO; GO:0045087; P:innate immune response; ISO:RGD.
DR   GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR   GO; GO:0051873; P:killing by host of symbiont cells; ISO:RGD.
DR   GO; GO:0031640; P:killing of cells of another organism; ISO:RGD.
DR   GO; GO:0051673; P:membrane disruption in another organism; IBA:GO_Central.
DR   GO; GO:1905710; P:positive regulation of membrane permeability; ISO:RGD.
DR   InterPro; IPR016327; Alpha-defensin.
DR   InterPro; IPR006081; Alpha-defensin_C.
DR   InterPro; IPR002366; Alpha-defensin_propep.
DR   InterPro; IPR006080; Defensin_beta/alpha.
DR   PANTHER; PTHR11876; PTHR11876; 1.
DR   Pfam; PF00323; Defensin_1; 1.
DR   Pfam; PF00879; Defensin_propep; 1.
DR   PIRSF; PIRSF001875; Alpha-defensin; 1.
DR   SMART; SM00048; DEFSN; 1.
DR   PROSITE; PS00269; DEFENSIN; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..62
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000006861"
FT   PEPTIDE         63..94
FT                   /note="Neutrophil antibiotic peptide NP-2"
FT                   /id="PRO_0000006862"
FT   DISULFID        65..93
FT                   /evidence="ECO:0000250"
FT   DISULFID        67..82
FT                   /evidence="ECO:0000250"
FT   DISULFID        72..92
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   94 AA;  10302 MW;  C379F26D98230E6C CRC64;
     MRTLTLLTAL LLLALHTQAK SPQGTAEEAP DQEQLVMEDQ DISISFGGDK GTALQDADVK
     AGVTCYCRST RCGFRERLSG ACGYRGRIYR LCCR