ID   DEF2_SORBI              Reviewed;          48 AA.
AC   P21924;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Defensin-like protein 2;
DE   AltName: Full=Small protein inhibitor of insect alpha-amylases 2;
DE            Short=SI alpha-2;
OS   Sorghum bicolor (Sorghum) (Sorghum vulgare).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Sorghinae; Sorghum.
OX   NCBI_TaxID=4558;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. French red; TISSUE=Seed;
RX   PubMed=1995329; DOI=10.1016/0014-5793(91)80261-z;
RA   Bloch C. Jr., Richardson M.;
RT   "A new family of small (5 kDa) protein inhibitors of insect alpha-amylases
RT   from seeds or sorghum (Sorghum bicolar (L) Moench) have sequence homologies
RT   with wheat gamma-purothionins.";
RL   FEBS Lett. 279:101-104(1991).
CC   -!- SIMILARITY: Belongs to the DEFL family. Protease inhibitor I18
CC       (RTI/MTI-2) subfamily. {ECO:0000305}.
CC   -!- CAUTION: Was initially thought to be a protease inhibitor.
CC       {ECO:0000305|PubMed:1995329}.
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DR   PIR; S13963; S13963.
DR   AlphaFoldDB; P21924; -.
DR   SMR; P21924; -.
DR   ExpressionAtlas; P21924; baseline.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   PRINTS; PR00288; PUROTHIONIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW   Plant defense.
FT   CHAIN           1..48
FT                   /note="Defensin-like protein 2"
FT                   /id="PRO_0000074249"
FT   DISULFID        3..47
FT                   /evidence="ECO:0000250"
FT   DISULFID        14..34
FT                   /evidence="ECO:0000250"
FT   DISULFID        20..41
FT                   /evidence="ECO:0000250"
FT   DISULFID        24..43
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   48 AA;  5316 MW;  37A43E1C9CA270BF CRC64;
     RVCMGKSAGF KGLCMRDQNC AQVCLQEGWG GGNCDGVMRQ CKCIRQCW