ID   DEF2_STOCA              Reviewed;          97 AA.
AC   O16137;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Defensin-2;
DE   Flags: Precursor;
GN   Name=SMD2;
OS   Stomoxys calcitrans (Stable fly) (Conops calcitrans).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC   Muscidae; Stomoxys.
OX   NCBI_TaxID=35570;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 58-67.
RC   TISSUE=Midgut;
RX   PubMed=9326639; DOI=10.1073/pnas.94.21.11502;
RA   Lehane M.J., Wu D., Lehane S.M.;
RT   "Midgut-specific immune molecules are produced by the blood-sucking insect
RT   Stomoxys calcitrans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:11502-11507(1997).
CC   -!- FUNCTION: Antibacterial peptide active mostly against Gram-negative
CC       bacteria. Its activity is enhanced by lipopolysaccharide.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Constitutively expressed in the anterior midgut and
CC       is up-regulated by blood feeding. Not expressed in the fat body or
CC       hemocytes.
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 1
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR   EMBL; AF013147; AAB66893.1; -; mRNA.
DR   RefSeq; NP_001298182.1; NM_001311253.1.
DR   AlphaFoldDB; O16137; -.
DR   STRING; 35570.O16137; -.
DR   GeneID; 106096091; -.
DR   VEuPathDB; VectorBase:SCAU015221; -.
DR   Proteomes; UP000095300; Genome assembly.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:UniProt.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR001542; Defensin_invertebrate/fungal.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   Pfam; PF01097; Defensin_2; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues; Defensin;
KW   Direct protein sequencing; Disulfide bond; Immunity; Innate immunity;
KW   Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   PROPEP          24..57
FT                   /evidence="ECO:0000269|PubMed:9326639"
FT                   /id="PRO_0000006752"
FT   CHAIN           58..97
FT                   /note="Defensin-2"
FT                   /id="PRO_0000006753"
FT   DISULFID        60..87
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT   DISULFID        73..93
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT   DISULFID        77..95
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
SQ   SEQUENCE   97 AA;  10614 MW;  BECC296E99A7FC4D CRC64;
     MKFFSLFPVI VVVVACLTMR ANAAPSAGNE VDHHPDYVDG VEALRQLEPE LHGRYKRATC
     DLLSMWNVNH SACAAHCLLL GKSGGRCNDD AVCVCRK