3BHS_PIG
ID 3BHS_PIG Reviewed; 373 AA.
AC Q9N119;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase;
DE Short=3-beta-HSD;
DE Includes:
DE RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE EC=1.1.1.145;
DE AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE AltName: Full=Progesterone reductase;
DE Includes:
DE RecName: Full=Steroid Delta-isomerase;
DE EC=5.3.3.1;
DE AltName: Full=Delta-5-3-ketosteroid isomerase;
GN Name=HSD3B {ECO:0000250|UniProtKB:P14893};
GN Synonyms=3b-HSD {ECO:0000312|EMBL:AAF37295.2};
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1] {ECO:0000312|EMBL:AAF37295.2}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Adipose tissue {ECO:0000312|EMBL:AAF37295.2};
RX PubMed=11683717; DOI=10.1046/j.1365-2052.2001.00775.x;
RA von Teichman A., Joerg H., Werner P., Brenig B., Stranzinger G.;
RT "cDNA cloning and physical mapping of porcine 3 beta-hydroxysteroid
RT dehydrogenase/Delta 5-delta 4 isomerase.";
RL Anim. Genet. 32:298-302(2001).
CC -!- FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the
CC oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the
CC oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system
CC plays a crucial role in the biosynthesis of all classes of hormonal
CC steroids.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-
CC steroid + H(+) + NADH; Xref=Rhea:RHEA:24076, ChEBI:CHEBI:1722,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47907, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.1.1.145;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid;
CC Xref=Rhea:RHEA:14709, ChEBI:CHEBI:47907, ChEBI:CHEBI:47909;
CC EC=5.3.3.1;
CC -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC membrane protein. Mitochondrion membrane; Single-pass membrane protein.
CC -!- SIMILARITY: Belongs to the 3-beta-HSD family. {ECO:0000305}.
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DR EMBL; AF232699; AAF37295.2; -; mRNA.
DR RefSeq; NP_001004049.1; NM_001004049.2.
DR AlphaFoldDB; Q9N119; -.
DR SMR; Q9N119; -.
DR STRING; 9823.ENSSSCP00000007165; -.
DR PeptideAtlas; Q9N119; -.
DR PRIDE; Q9N119; -.
DR Ensembl; ENSSSCT00000007360; ENSSSCP00000007165; ENSSSCG00000006719.
DR Ensembl; ENSSSCT00025040671; ENSSSCP00025017317; ENSSSCG00025029902.
DR Ensembl; ENSSSCT00030043165; ENSSSCP00030019583; ENSSSCG00030031100.
DR Ensembl; ENSSSCT00035094806; ENSSSCP00035039835; ENSSSCG00035070172.
DR Ensembl; ENSSSCT00045003183; ENSSSCP00045001999; ENSSSCG00045002042.
DR Ensembl; ENSSSCT00055002747; ENSSSCP00055002074; ENSSSCG00055001503.
DR Ensembl; ENSSSCT00060048493; ENSSSCP00060020760; ENSSSCG00060035784.
DR Ensembl; ENSSSCT00070006206; ENSSSCP00070005059; ENSSSCG00070003287.
DR GeneID; 445539; -.
DR KEGG; ssc:445539; -.
DR CTD; 3283; -.
DR eggNOG; KOG1430; Eukaryota.
DR GeneTree; ENSGT00940000155444; -.
DR HOGENOM; CLU_007383_6_3_1; -.
DR InParanoid; Q9N119; -.
DR OMA; SLEDCRG; -.
DR OrthoDB; 930591at2759; -.
DR TreeFam; TF343138; -.
DR BRENDA; 1.1.1.145; 6170.
DR Reactome; R-SSC-193048; Androgen biosynthesis.
DR Reactome; R-SSC-193993; Mineralocorticoid biosynthesis.
DR Reactome; R-SSC-194002; Glucocorticoid biosynthesis.
DR SABIO-RK; Q9N119; -.
DR UniPathway; UPA00062; -.
DR Proteomes; UP000008227; Chromosome 4.
DR Proteomes; UP000314985; Chromosome 4.
DR Bgee; ENSSSCG00000006719; Expressed in ovary and 21 other tissues.
DR ExpressionAtlas; Q9N119; baseline and differential.
DR Genevisible; Q9N119; SS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0102294; F:cholesterol dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR GO; GO:0004769; F:steroid delta-isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0008207; P:C21-steroid hormone metabolic process; IBA:GO_Central.
DR GO; GO:0021766; P:hippocampus development; IBA:GO_Central.
DR GO; GO:0051412; P:response to corticosterone; IBA:GO_Central.
DR GO; GO:0006694; P:steroid biosynthetic process; IBA:GO_Central.
DR InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01073; 3Beta_HSD; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion;
KW Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome;
KW Steroidogenesis; Transmembrane; Transmembrane helix.
FT CHAIN 1..373
FT /note="3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-
FT isomerase"
FT /id="PRO_0000087786"
FT TRANSMEM 292..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 155
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 159
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 373 AA; 41882 MW; 8BD4FEEF0117F6FF CRC64;
MAGWSCLVTG GGGFLGQRIV HLLLEEKDLQ EIRVLDKVFK PEVREEFSKL QSKIKLTMLE
GDILDEQCLK GACQGASVVI HTASIIDVVN AVGRETVMKV NVKGTQLLLE ACVQASVPVF
IHTSSIEVAG PNSYREVIQN ACEEDRLETA WSAPYPLSKK LAEKAVLEAN GWALQNGGTL
HTCALRPMYI YGEGSPFIFA HMNKALENNG VLTHNSKFSR VNPVYVGNVA WAHILALRAL
RDPRKALSVQ GQFYYVADDT PPQSYDDLNY TLGKEWGFCL DSRRSLPPSL RYWLAFLLEI
VSFLLSPIYN YQPPFNRHFV TLCNSVFTVS YKKAQRDLGY EPLFTWEEAK QKTKAWVGSL
VKQHKEALKT KTH
