DEF2_WHEAT
ID DEF2_WHEAT Reviewed; 47 AA.
AC P20159;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 87.
DE RecName: Full=Defensin-like protein 2;
DE AltName: Full=Gamma-2-purothionin;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP PROTEIN SEQUENCE, AND FUNCTION.
RC STRAIN=cv. Senatore Capelli; TISSUE=Endosperm;
RX PubMed=2226781; DOI=10.1016/0014-5793(90)81265-p;
RA Colilla F.J., Rocher A., Mendez E.;
RT "Gamma-purothionins: amino acid sequence of two polypeptides of a new
RT family of thionins from wheat endosperm.";
RL FEBS Lett. 270:191-194(1990).
CC -!- FUNCTION: Inhibits protein translation in cell-free systems
CC (PubMed:2226781). May inhibit trypsin (By similarity).
CC {ECO:0000250|UniProtKB:P83399, ECO:0000269|PubMed:2226781}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC -!- CAUTION: Was initially thought to be a thionin.
CC {ECO:0000305|PubMed:2226781}.
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DR AlphaFoldDB; P20159; -.
DR SMR; P20159; -.
DR TCDB; 1.C.45.1.3; the plant defensin (plant defensin) family.
DR EnsemblPlants; TraesCAD_scaffold_010255_01G000200.1; TraesCAD_scaffold_010255_01G000200.1; TraesCAD_scaffold_010255_01G000200.
DR EnsemblPlants; TraesCLE_scaffold_013587_01G000200.1; TraesCLE_scaffold_013587_01G000200.1; TraesCLE_scaffold_013587_01G000200.
DR EnsemblPlants; TraesPAR_scaffold_012933_01G000200.1; TraesPAR_scaffold_012933_01G000200.1; TraesPAR_scaffold_012933_01G000200.
DR EnsemblPlants; TraesROB_scaffold_167115_01G000200.1; TraesROB_scaffold_167115_01G000200.1; TraesROB_scaffold_167115_01G000200.
DR EnsemblPlants; TraesWEE_scaffold_011003_01G000200.1; TraesWEE_scaffold_011003_01G000200.1; TraesWEE_scaffold_011003_01G000200.
DR Gramene; TraesCAD_scaffold_010255_01G000200.1; TraesCAD_scaffold_010255_01G000200.1; TraesCAD_scaffold_010255_01G000200.
DR Gramene; TraesCLE_scaffold_013587_01G000200.1; TraesCLE_scaffold_013587_01G000200.1; TraesCLE_scaffold_013587_01G000200.
DR Gramene; TraesPAR_scaffold_012933_01G000200.1; TraesPAR_scaffold_012933_01G000200.1; TraesPAR_scaffold_012933_01G000200.
DR Gramene; TraesROB_scaffold_167115_01G000200.1; TraesROB_scaffold_167115_01G000200.1; TraesROB_scaffold_167115_01G000200.
DR Gramene; TraesWEE_scaffold_011003_01G000200.1; TraesWEE_scaffold_011003_01G000200.1; TraesWEE_scaffold_011003_01G000200.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; P20159; baseline and differential.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR PRINTS; PR00288; PUROTHIONIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 1: Evidence at protein level;
KW Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW Plant defense; Reference proteome.
FT CHAIN 1..47
FT /note="Defensin-like protein 2"
FT /id="PRO_0000074262"
FT SITE 11
FT /note="Interaction with trypsin"
FT /evidence="ECO:0000250|UniProtKB:P83399"
FT DISULFID 3..47
FT /evidence="ECO:0000250|UniProtKB:P21925"
FT DISULFID 14..34
FT /evidence="ECO:0000250|UniProtKB:P21925"
FT DISULFID 20..41
FT /evidence="ECO:0000250|UniProtKB:P21925"
FT DISULFID 24..43
FT /evidence="ECO:0000250|UniProtKB:P21925"
SQ SEQUENCE 47 AA; 5151 MW; 93EF21C1BCA79300 CRC64;
KVCRQRSAGF KGPCVSDKNC AQVCLQEGWG GGNCDGPFRR CKCIRQC
