ID   DEF3B_RAT               Reviewed;          87 AA.
AC   Q9Z1F1;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Neutrophil antibiotic peptide NP-3B;
DE            Short=RatNP-3b;
DE   AltName: Full=Neutrophil defensin 3;
DE   Flags: Precursor;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Neutrophil;
RA   Banaiee N., Yount N.Y., Selsted M.E.;
RT   "Molecular characterization of genes encoding rat neutrophil defensins.";
RL   Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 59-87.
RC   STRAIN=Sprague-Dawley; TISSUE=Peritoneal neutrophil;
RX   PubMed=2543629; DOI=10.1128/iai.57.7.2021-2027.1989;
RA   Eisenhauer P.B., Harwig S.S.S.L., Szklarek D., Ganz T., Selsted M.E.,
RA   Lehrer R.I.;
RT   "Purification and antimicrobial properties of three defensins from rat
RT   neutrophils.";
RL   Infect. Immun. 57:2021-2027(1989).
CC   -!- FUNCTION: Active in vitro against S.aureus, fungi, Gram-positive and
CC       Gram-negative bacteria and to a lesser extent against an enveloped
CC       virus.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
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DR   EMBL; U50354; AAC99550.1; -; Genomic_DNA.
DR   PIR; B61014; B61014.
DR   RefSeq; NP_001073367.2; NM_001079898.2.
DR   AlphaFoldDB; Q9Z1F1; -.
DR   SMR; Q9Z1F1; -.
DR   STRING; 10116.ENSRNOP00000018580; -.
DR   PaxDb; Q9Z1F1; -.
DR   Ensembl; ENSRNOT00000018580; ENSRNOP00000018580; ENSRNOG00000038135.
DR   GeneID; 498659; -.
DR   KEGG; rno:498659; -.
DR   UCSC; RGD:1589653; rat.
DR   CTD; 498659; -.
DR   GeneTree; ENSGT00940000153268; -.
DR   HOGENOM; CLU_160803_3_0_1; -.
DR   InParanoid; Q9Z1F1; -.
DR   OMA; RARIICH; -.
DR   OrthoDB; 1610714at2759; -.
DR   Reactome; R-RNO-1461973; Defensins.
DR   Reactome; R-RNO-1462054; Alpha-defensins.
DR   Reactome; R-RNO-6798695; Neutrophil degranulation.
DR   PRO; PR:Q9Z1F1; -.
DR   Proteomes; UP000002494; Chromosome 16.
DR   Bgee; ENSRNOG00000038135; Expressed in thymus and 14 other tissues.
DR   Genevisible; Q9Z1F1; RN.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR   GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IBA:GO_Central.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IBA:GO_Central.
DR   GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   GO; GO:0051673; P:membrane disruption in another organism; IBA:GO_Central.
DR   InterPro; IPR016327; Alpha-defensin.
DR   InterPro; IPR006081; Alpha-defensin_C.
DR   InterPro; IPR002366; Alpha-defensin_propep.
DR   InterPro; IPR006080; Defensin_beta/alpha.
DR   PANTHER; PTHR11876; PTHR11876; 1.
DR   Pfam; PF00323; Defensin_1; 1.
DR   Pfam; PF00879; Defensin_propep; 1.
DR   PIRSF; PIRSF001875; Alpha-defensin; 1.
DR   SMART; SM00048; DEFSN; 1.
DR   PROSITE; PS00269; DEFENSIN; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..58
FT                   /evidence="ECO:0000269|PubMed:2543629"
FT                   /id="PRO_0000006865"
FT   PEPTIDE         59..87
FT                   /note="Neutrophil antibiotic peptide NP-3B"
FT                   /id="PRO_0000006866"
FT   DISULFID        59..87
FT                   /evidence="ECO:0000250"
FT   DISULFID        61..76
FT                   /evidence="ECO:0000250"
FT   DISULFID        66..86
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   87 AA;  9353 MW;  1A8CFD0D164EE5C5 CRC64;
     MRTLILLTTL LLLALHTQAE SPQGSTKEAP DEEQDISVFF GGDKGTALQD AAVKAGVTCS
     CRTSSCRFGE RLSGACRLNG RIYRLCC