DEF3_ANOGA
ID DEF3_ANOGA Reviewed; 67 AA.
AC Q5TWR9; Q2KM07;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 25-MAY-2022, entry version 70.
DE RecName: Full=Antimicrobial peptide defensin 3 {ECO:0000312|EMBL:AAX92637.1};
DE Short=AgDef3 {ECO:0000303|PubMed:18353100};
DE Flags: Precursor;
GN Name=Def3 {ECO:0000312|EMBL:EAL41851.2}; ORFNames=AGAP007199;
OS Anopheles gambiae (African malaria mosquito).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=7165;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAX92637.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION.
RC STRAIN=Keele {ECO:0000312|EMBL:AAX92637.1};
RC TISSUE=Larva {ECO:0000269|PubMed:18353100};
RX PubMed=18353100; DOI=10.1111/j.1365-2583.2008.00786.x;
RA Meredith J.M., Hurd H., Lehane M.J., Eggleston P.;
RT "The malaria vector mosquito Anopheles gambiae expresses a suite of larval-
RT specific defensin genes.";
RL Insect Mol. Biol. 17:103-112(2008).
RN [2] {ECO:0000312|EMBL:EAL41851.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PEST;
RX PubMed=12364791; DOI=10.1126/science.1076181;
RA Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R.,
RA Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R.,
RA Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z.,
RA Kraft C.L., Abril J.F., Anthouard V., Arensburger P., Atkinson P.W.,
RA Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C.,
RA Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K.,
RA Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V.,
RA Dana A., Delcher A., Dew I., Evans C.A., Flanigan M.,
RA Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R.,
RA Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J.,
RA Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I.,
RA Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A.,
RA McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D.,
RA O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H.,
RA Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J.,
RA Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B.,
RA Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M.,
RA Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I.,
RA Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J.,
RA Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M.,
RA Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C.,
RA Collins F.H., Hoffman S.L.;
RT "The genome sequence of the malaria mosquito Anopheles gambiae.";
RL Science 298:129-149(2002).
RN [3] {ECO:0000305}
RP IDENTIFICATION.
RX PubMed=16835022; DOI=10.1016/j.ibmb.2006.04.005;
RA Calvo E., Pham V.M., Lombardo F., Arca B., Ribeiro J.M.;
RT "The sialotranscriptome of adult male Anopheles gambiae mosquitoes.";
RL Insect Biochem. Mol. Biol. 36:570-575(2006).
CC -!- FUNCTION: Antibacterial peptide mostly active against Gram-positive
CC bacteria with atypical up-regulation of expression.
CC {ECO:0000255|PROSITE-ProRule:PRU00710, ECO:0000269|PubMed:18353100}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00710}.
CC -!- DEVELOPMENTAL STAGE: Basal levels of expression during all life stages,
CC with higher levels during larval development peaking at larval instars
CC 2/3. {ECO:0000269|PubMed:18353100}.
CC -!- INDUCTION: Not induced in 4th instar larvae following a blood meal, an
CC infected blood meal, sterile injection or bacterial injection (E.coli
CC K12 RM148 and M.luteus). {ECO:0000269|PubMed:18353100}.
CC -!- SIMILARITY: Belongs to the invertebrate defensin family.
CC {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR EMBL; AY907825; AAX92637.1; -; mRNA.
DR EMBL; AAAB01008807; EAL41851.2; -; Genomic_DNA.
DR RefSeq; XP_565009.2; XM_565009.2.
DR AlphaFoldDB; Q5TWR9; -.
DR SMR; Q5TWR9; -.
DR STRING; 7165.AGAP007199-PA; -.
DR PaxDb; Q5TWR9; -.
DR GeneID; 3290326; -.
DR KEGG; aga:AgaP_AGAP007199; -.
DR CTD; 3290326; -.
DR VEuPathDB; VectorBase:AGAP007199; -.
DR HOGENOM; CLU_2814504_0_0_1; -.
DR OrthoDB; 1615816at2759; -.
DR Proteomes; UP000007062; Chromosome 2L.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0042742; P:defense response to bacterium; IMP:UniProtKB.
DR InterPro; IPR001542; Defensin_invertebrate/fungal.
DR PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE 2: Evidence at transcript level;
KW Antibiotic; Antimicrobial; Defensin; Disulfide bond; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..67
FT /note="Antimicrobial peptide defensin 3"
FT /evidence="ECO:0000255"
FT /id="PRO_0000398808"
FT DISULFID 29..56
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT DISULFID 42..62
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT DISULFID 46..64
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT CONFLICT 67
FT /note="T -> A (in Ref. 1; AAX92637)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 67 AA; 6954 MW; CD6DB1EB2C66C983 CRC64;
MKFAVVSFLL VALLGLVAVG EAQLKNLACV TNEGPKWANT YCAAVCHMSG RGAGSCNAKD
ECVCSMT
