ID   DEF3_BRANA              Reviewed;          79 AA.
AC   Q39313;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Defensin-like protein 3;
DE   AltName: Full=Cysteine-rich antifungal protein 3;
DE            Short=AFP3;
DE   Flags: Precursor;
GN   Name=AFP3;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Naehan;
RA   Sohn U., Lee C.M., Lee M.H., Kim J.H.;
RL   Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Possesses antifungal activity sensitive to inorganic cations.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR   EMBL; U59459; AAB03224.1; -; mRNA.
DR   PIR; T07917; T07917.
DR   RefSeq; XP_013717975.1; XM_013862521.1.
DR   AlphaFoldDB; Q39313; -.
DR   SMR; Q39313; -.
DR   EnsemblPlants; CDX87658; CDX87658; GSBRNA2T00146665001.
DR   GeneID; 106421680; -.
DR   Gramene; CDX87658; CDX87658; GSBRNA2T00146665001.
DR   KEGG; bna:106421680; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   3: Inferred from homology;
KW   Antimicrobial; Disulfide bond; Fungicide; Plant defense; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..79
FT                   /note="Defensin-like protein 3"
FT                   /id="PRO_0000007035"
FT   DISULFID        32..79
FT                   /evidence="ECO:0000250"
FT   DISULFID        43..64
FT                   /evidence="ECO:0000250"
FT   DISULFID        49..73
FT                   /evidence="ECO:0000250"
FT   DISULFID        53..75
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   79 AA;  8555 MW;  3B5289FCFEA48936 CRC64;
     MAKFASIITL LFAALVVFAA FEAPTMVEAK LCERSSGTWS GVCGNNNACK NQCIRLEGAQ
     HGSCNYVFPA HKCICYFPC