ID   DEF3_RAPSA              Reviewed;          79 AA.
AC   O24332;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Defensin-like protein 3;
DE   AltName: Full=Cysteine-rich antifungal protein 3;
DE            Short=AFP3;
DE   Flags: Precursor;
GN   Name=AFP3;
OS   Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus.
OX   NCBI_TaxID=3726;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Ronde Rode Kleine Witpunt; TISSUE=Seed;
RA   Terras F.R.G., Goderis I.J., Penninckx I.J., Osborn R.W., Broekaert W.F.;
RL   Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Possesses antifungal activity sensitive to inorganic cations.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR   EMBL; X97319; CAA65984.1; -; mRNA.
DR   PIR; T10243; T10243.
DR   AlphaFoldDB; O24332; -.
DR   SMR; O24332; -.
DR   Proteomes; UP000504610; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   3: Inferred from homology;
KW   Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..79
FT                   /note="Defensin-like protein 3"
FT                   /id="PRO_0000007053"
FT   DISULFID        32..79
FT                   /evidence="ECO:0000250"
FT   DISULFID        43..64
FT                   /evidence="ECO:0000250"
FT   DISULFID        49..73
FT                   /evidence="ECO:0000250"
FT   DISULFID        53..75
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   79 AA;  8479 MW;  BAFA80465DB48548 CRC64;
     MAKFASIVAL LFAALVVFAA FEAPTVVEAK LCERSSGTWS GVCGNNNACK NQCIRLEGAQ
     HGSCNYVFPA HKCICYFPC