ID   DEF4_BRANA              Reviewed;          60 AA.
AC   P80301;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Defensin-like protein 4;
DE   AltName: Full=RTI;
DE   AltName: Full=Trypsin inhibitor;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=cv. Oleifera; TISSUE=Seed;
RX   PubMed=8143882; DOI=10.1016/0014-5793(94)80505-9;
RA   Ceciliani F., Menegatti E., Ronchi S., Bortolotti F., Ascenzi P.,
RA   Palmieri S.;
RT   "Purification, inhibitory properties, amino acid sequence and
RT   identification of the reactive site of a new serine proteinase inhibitor
RT   from oil-rape (Brassica napus) seed.";
RL   FEBS Lett. 342:221-224(1994).
CC   -!- FUNCTION: Inhibits trypsin and chymotrypsin.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the DEFL family. Protease inhibitor I18
CC       (RTI/MTI-2) subfamily. {ECO:0000305}.
CC   -!- CAUTION: Was initially thought to be a protease inhibitor.
CC       {ECO:0000305|PubMed:8143882}.
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DR   PIR; S43672; S43672.
DR   AlphaFoldDB; P80301; -.
DR   SMR; P80301; -.
DR   MEROPS; I18.002; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR002061; Scorpion_toxinL/defensin.
DR   Pfam; PF00537; Toxin_3; 1.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW   Plant defense; Secreted.
FT   CHAIN           1..60
FT                   /note="Defensin-like protein 4"
FT                   /id="PRO_0000221426"
FT   SITE            20..21
FT                   /note="Reactive bond"
FT   DISULFID        4..56
FT                   /evidence="ECO:0000250"
FT   DISULFID        17..41
FT                   /evidence="ECO:0000250"
FT   DISULFID        26..51
FT                   /evidence="ECO:0000250"
FT   DISULFID        30..53
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   60 AA;  6609 MW;  01F94231016D6CC6 CRC64;
     DSECLKEYGG DVGFGFCAPR IYPSFCVQRC RADKGALSGK CIWGQGSNVK CLCNFCRHEP