ID   DEF4_RAPSA              Reviewed;          80 AA.
AC   O24331;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Defensin-like protein 4;
DE   AltName: Full=Cysteine-rich antifungal protein 4;
DE            Short=AFP4;
DE   Flags: Precursor;
GN   Name=AFP4;
OS   Raphanus sativus (Radish) (Raphanus raphanistrum var. sativus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Raphanus.
OX   NCBI_TaxID=3726;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Ronde Rode Kleine Witpunt; TISSUE=Seed;
RA   Terras F.R.G., Goderis I.J., Penninckx I.J., Osborn R.W., Broekaert W.F.;
RL   Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Possesses antifungal activity sensitive to inorganic cations.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR   EMBL; X97318; CAA65983.1; -; mRNA.
DR   PIR; T10183; T10183.
DR   RefSeq; XP_018443188.1; XM_018587686.1.
DR   AlphaFoldDB; O24331; -.
DR   SMR; O24331; -.
DR   GeneID; 108815027; -.
DR   KEGG; rsz:108815027; -.
DR   OrthoDB; 1565340at2759; -.
DR   Proteomes; UP000504610; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   3: Inferred from homology;
KW   Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW   Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..80
FT                   /note="Defensin-like protein 4"
FT                   /id="PRO_0000007054"
FT   MOD_RES         30
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P30231"
FT   DISULFID        33..80
FT                   /evidence="ECO:0000250"
FT   DISULFID        44..65
FT                   /evidence="ECO:0000250"
FT   DISULFID        50..74
FT                   /evidence="ECO:0000250"
FT   DISULFID        54..76
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   80 AA;  8873 MW;  B5F667B6441818C9 CRC64;
     MAKFVSIITL LFVALVLFAA FEAPTMVEAQ KLCERSSGTW SGVCGNNNAC KNQCINLEGA
     RHGSCNYIFP YHRCICYFPC