DEF4_RAT
ID DEF4_RAT Reviewed; 93 AA.
AC Q62714;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Defensin alpha 4 {ECO:0000250|UniProtKB:P12838};
DE AltName: Full=Neutrophil antibiotic peptide NP-4 {ECO:0000303|PubMed:7594610};
DE Short=RatNP-4 {ECO:0000303|PubMed:7594610};
DE AltName: Full=Neutrophil defensin 4 {ECO:0000303|PubMed:7594610};
DE Flags: Precursor;
GN Name=Np4 {ECO:0000303|PubMed:7594610};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Bone marrow;
RX PubMed=7594610;
RA Yount N.Y., Wang M.-S.C., Yuan J., Banaiee N., Ouellette A.J.,
RA Selsted M.E.;
RT "Rat neutrophil defensins. Precursor structures and expression during
RT neutrophilic myelopoiesis.";
RL J. Immunol. 155:4476-4484(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Sprague-Dawley; TISSUE=Neutrophil;
RA Banaiee N., Yount N.Y., Selsted M.E.;
RT "Molecular characterization of genes encoding rat neutrophil defensins.";
RL Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP PROTEIN SEQUENCE OF 63-93, FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Peritoneal neutrophil;
RX PubMed=2543629; DOI=10.1128/iai.57.7.2021-2027.1989;
RA Eisenhauer P.B., Harwig S.S.S.L., Szklarek D., Ganz T., Selsted M.E.,
RA Lehrer R.I.;
RT "Purification and antimicrobial properties of three defensins from rat
RT neutrophils.";
RL Infect. Immun. 57:2021-2027(1989).
CC -!- FUNCTION: Host-defense peptide that has antimicrobial activity against
CC Gram-positive and Gram-negative bacteria and fungi (in vitro)
CC (PubMed:2543629). Exhibits activity against E.coli, A.calcoaceticus,
CC S,aureus and C.albicans (PubMed:2543629). {ECO:0000269|PubMed:2543629}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q01523}.
CC -!- TISSUE SPECIFICITY: Expressed in neutrophils (at protein level)
CC (PubMed:2543629). Highest expression in bone marrow and to a much
CC lesser extent in small intestine (PubMed:7594610).
CC {ECO:0000269|PubMed:2543629, ECO:0000269|PubMed:7594610}.
CC -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U16684; AAA91972.1; -; mRNA.
DR EMBL; U50355; AAC99552.1; -; Genomic_DNA.
DR PIR; C60113; A61014.
DR RefSeq; NP_775421.1; NM_173299.2.
DR AlphaFoldDB; Q62714; -.
DR SMR; Q62714; -.
DR STRING; 10116.ENSRNOP00000036962; -.
DR PaxDb; Q62714; -.
DR PRIDE; Q62714; -.
DR GeneID; 286958; -.
DR KEGG; rno:286958; -.
DR UCSC; RGD:727857; rat.
DR CTD; 286958; -.
DR RGD; 727857; Np4.
DR VEuPathDB; HostDB:ENSRNOG00000069755; -.
DR HOGENOM; CLU_160803_3_0_1; -.
DR InParanoid; Q62714; -.
DR OrthoDB; 1610714at2759; -.
DR PhylomeDB; Q62714; -.
DR TreeFam; TF338414; -.
DR Reactome; R-RNO-1461973; Defensins.
DR Reactome; R-RNO-1462054; Alpha-defensins.
DR Reactome; R-RNO-6798695; Neutrophil degranulation.
DR PRO; PR:Q62714; -.
DR Proteomes; UP000002494; Chromosome 16.
DR Bgee; ENSRNOG00000028707; Expressed in thymus and 10 other tissues.
DR ExpressionAtlas; Q62714; baseline and differential.
DR Genevisible; Q62714; RN.
DR GO; GO:0042582; C:azurophil granule; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; TAS:RGD.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0019731; P:antibacterial humoral response; ISO:RGD.
DR GO; GO:0019732; P:antifungal humoral response; ISO:RGD.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; ISO:RGD.
DR GO; GO:0019730; P:antimicrobial humoral response; ISO:RGD.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; TAS:RGD.
DR GO; GO:0042742; P:defense response to bacterium; ISO:RGD.
DR GO; GO:0050832; P:defense response to fungus; ISO:RGD.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; ISO:RGD.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; ISO:RGD.
DR GO; GO:0045087; P:innate immune response; ISO:RGD.
DR GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR GO; GO:0051673; P:membrane disruption in another organism; IBA:GO_Central.
DR InterPro; IPR016327; Alpha-defensin.
DR InterPro; IPR006081; Alpha-defensin_C.
DR InterPro; IPR002366; Alpha-defensin_propep.
DR InterPro; IPR006080; Defensin_beta/alpha.
DR PANTHER; PTHR11876; PTHR11876; 1.
DR Pfam; PF00323; Defensin_1; 1.
DR Pfam; PF00879; Defensin_propep; 1.
DR PIRSF; PIRSF001875; Alpha-defensin; 1.
DR SMART; SM00048; DEFSN; 1.
DR PROSITE; PS00269; DEFENSIN; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW Disulfide bond; Fungicide; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..62
FT /evidence="ECO:0000255"
FT /id="PRO_0000006867"
FT PEPTIDE 63..93
FT /note="Defensin alpha 4"
FT /id="PRO_0000006868"
FT DISULFID 64..92
FT /evidence="ECO:0000250|UniProtKB:P28311"
FT DISULFID 66..81
FT /evidence="ECO:0000250|UniProtKB:P28311"
FT DISULFID 71..91
FT /evidence="ECO:0000250|UniProtKB:P28311"
SQ SEQUENCE 93 AA; 10110 MW; A768917212603137 CRC64;
MRTLTLLITL LLLALHTQAE SPQERAKAAP DQDMVMEDQD IFISFGGYKG TVLQDAVVKA
GQACYCRIGA CVSGERLTGA CGLNGRIYRL CCR
