DEFA1_MOUSE
ID DEFA1_MOUSE Reviewed; 93 AA.
AC P11477; Q61448;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1990, sequence version 2.
DT 25-MAY-2022, entry version 144.
DE RecName: Full=Alpha-defensin 1;
DE AltName: Full=Cryptdin-1;
DE AltName: Full=Defensin-related cryptdin peptide;
DE Flags: Precursor;
GN Name=Defa1; Synonyms=Defcr, Defcr1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Swiss albino; TISSUE=Small intestine;
RX PubMed=2351676; DOI=10.1016/s0021-9258(19)38746-0;
RA Ouellette A.J., Lauldi J.C.;
RT "A novel mouse gene family coding for cationic, cysteine-rich peptides.
RT Regulation in small intestine and cells of myeloid origin.";
RL J. Biol. Chem. 265:9831-9837(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129;
RX PubMed=8188287; DOI=10.1006/geno.1994.1093;
RA Huttner K.M., Selsted M.E., Ouellette A.J.;
RT "Structure and diversity of the murine cryptdin gene family.";
RL Genomics 19:448-453(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2715173; DOI=10.1083/jcb.108.5.1687;
RA Ouellette A.J., Greco R.M., James M., Frederick D., Naftilan J.,
RA Fallon J.T.;
RT "Developmental regulation of cryptdin, a corticostatin/defensin precursor
RT mRNA in mouse small intestinal crypt epithelium.";
RL J. Cell Biol. 108:1687-1695(1989).
RN [4]
RP PROTEIN SEQUENCE OF 59-93.
RC STRAIN=SWR/J; TISSUE=Small intestine;
RX PubMed=1500431; DOI=10.1083/jcb.118.4.929;
RA Selsted M.E., Miller S.I., Henschen A.H., Ouellette A.J.;
RT "Enteric defensins: antibiotic peptide components of intestinal host
RT defense.";
RL J. Cell Biol. 118:929-936(1992).
RN [5]
RP PROTEIN SEQUENCE OF 59-93.
RC STRAIN=SWR/J; TISSUE=Intestinal crypt;
RX PubMed=1618314; DOI=10.1016/0014-5793(92)80606-h;
RA Ouellette A.J., Miller S.I., Henschen A.H., Selsted M.E.;
RT "Purification and primary structure of murine cryptdin-1, a Paneth cell
RT defensin.";
RL FEBS Lett. 304:146-148(1992).
CC -!- FUNCTION: Probably contributes to the antimicrobial barrier function of
CC the small bowel mucosa. Has antibacterial activity against attenuated
CC mutants of S.typhimurium.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Paneth cells of the small bowel.
CC -!- DEVELOPMENTAL STAGE: Accumulates to high levels in mouse intestinal
CC crypt epithelium during postnatal development.
CC -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
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DR EMBL; M33225; AAA63296.1; -; mRNA.
DR EMBL; U02995; AAB60675.1; -; Genomic_DNA.
DR EMBL; U02994; AAB60675.1; JOINED; Genomic_DNA.
DR EMBL; X15617; CAA33636.1; -; mRNA.
DR PIR; A36438; A43279.
DR RefSeq; NP_034161.2; NM_010031.2.
DR AlphaFoldDB; P11477; -.
DR SMR; P11477; -.
DR PhosphoSitePlus; P11477; -.
DR PeptideAtlas; P11477; -.
DR PRIDE; P11477; -.
DR DNASU; 13216; -.
DR GeneID; 13216; -.
DR KEGG; mmu:13216; -.
DR CTD; 1667; -.
DR MGI; MGI:94880; Defa1.
DR InParanoid; P11477; -.
DR PhylomeDB; P11477; -.
DR PRO; PR:P11477; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P11477; protein.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IGI:MGI.
DR GO; GO:0042742; P:defense response to bacterium; IDA:MGI.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IGI:MGI.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IGI:MGI.
DR GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR GO; GO:0051673; P:membrane disruption in another organism; IBA:GO_Central.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IDA:MGI.
DR InterPro; IPR016327; Alpha-defensin.
DR InterPro; IPR006081; Alpha-defensin_C.
DR InterPro; IPR002366; Alpha-defensin_propep.
DR InterPro; IPR006080; Defensin_beta/alpha.
DR PANTHER; PTHR11876; PTHR11876; 1.
DR Pfam; PF00323; Defensin_1; 1.
DR Pfam; PF00879; Defensin_propep; 1.
DR PIRSF; PIRSF001875; Alpha-defensin; 1.
DR SMART; SM00048; DEFSN; 1.
DR PROSITE; PS00269; DEFENSIN; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW Disulfide bond; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..58
FT /evidence="ECO:0000255"
FT /id="PRO_0000006817"
FT PEPTIDE 59..93
FT /note="Alpha-defensin 1"
FT /id="PRO_0000006818"
FT REGION 24..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 64..92
FT /evidence="ECO:0000250"
FT DISULFID 66..81
FT /evidence="ECO:0000250"
FT DISULFID 71..91
FT /evidence="ECO:0000250"
FT CONFLICT 3
FT /note="K -> T (in Ref. 2; AAB60675)"
FT /evidence="ECO:0000305"
FT CONFLICT 8
FT /note="F -> S (in Ref. 2; AAB60675)"
FT /evidence="ECO:0000305"
FT CONFLICT 14
FT /note="G -> A (in Ref. 2; AAB60675)"
FT /evidence="ECO:0000305"
FT CONFLICT 21
FT /note="S -> P (in Ref. 2; AAB60675)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 93 AA; 10461 MW; 3CD0B6FB9644A22D CRC64;
MKKLVLLFAL VLLGFQVQAD SIQNTDEETK TEEQPGEEDQ AVSVSFGDPE GTSLQEESLR
DLVCYCRSRG CKGRERMNGT CRKGHLLYTL CCR
