DEFA2_MOUSE
ID DEFA2_MOUSE Reviewed; 93 AA.
AC P28309; P82293; Q60616; Q62537;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Alpha-defensin 2;
DE AltName: Full=Cryptdin-1;
DE AltName: Full=Defensin-related cryptdin-2;
DE Flags: Precursor;
GN Name=Defa2; Synonyms=Defcr2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129; TISSUE=Small intestine;
RX PubMed=8188287; DOI=10.1006/geno.1994.1093;
RA Huttner K.M., Selsted M.E., Ouellette A.J.;
RT "Structure and diversity of the murine cryptdin gene family.";
RL Genomics 19:448-453(1994).
RN [2]
RP PROTEIN SEQUENCE OF 59-93.
RC STRAIN=SWR/J; TISSUE=Small intestine;
RX PubMed=1500431; DOI=10.1083/jcb.118.4.929;
RA Selsted M.E., Miller S.I., Henschen A.H., Ouellette A.J.;
RT "Enteric defensins: antibiotic peptide components of intestinal host
RT defense.";
RL J. Cell Biol. 118:929-936(1992).
RN [3]
RP PROTEIN SEQUENCE OF 59-93, AND FUNCTION.
RC STRAIN=Swiss Webster; TISSUE=Small intestine;
RX PubMed=1500163; DOI=10.1128/iai.60.9.3556-3565.1992;
RA Eisenhauer P.B., Harwig S.S.S.L., Lehrer R.I.;
RT "Cryptdins: antimicrobial defensins of the murine small intestine.";
RL Infect. Immun. 60:3556-3565(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-93.
RC STRAIN=C57BL/6J;
RX PubMed=8043949; DOI=10.1007/bf00356553;
RA Ko M.S., Wang X., Horton J.H., Hagen M.D., Takahashi N., Maezaki Y.,
RA Nadeau J.H.;
RT "Genetic mapping of 40 cDNA clones on the mouse genome by PCR.";
RL Mamm. Genome 5:349-355(1994).
CC -!- FUNCTION: Has broad-spectrum antimicrobial properties. Has
CC antibacterial activity against the Gram-positive bacterium
CC L.monocytogenes EGD and the Gram-negative bacteria E.coli ML-35p and
CC avirulent S.typhimurium 7953, but not against the mouse-virulent
CC S.typhimurium 14028S. Probably contributes to the antimicrobial barrier
CC function of the small bowel mucosa. {ECO:0000269|PubMed:1500163}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Paneth cells of the small bowel.
CC -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
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DR EMBL; U02997; AAB60676.1; -; Genomic_DNA.
DR EMBL; U02996; AAB60676.1; JOINED; Genomic_DNA.
DR EMBL; U03028; AAA57170.1; -; mRNA.
DR EMBL; U05705; AAB60468.1; -; Genomic_DNA.
DR PIR; I48226; I48226.
DR PIR; I49413; I49413.
DR AlphaFoldDB; P28309; -.
DR SMR; P28309; -.
DR iPTMnet; P28309; -.
DR PhosphoSitePlus; P28309; -.
DR PeptideAtlas; P28309; -.
DR PRIDE; P28309; -.
DR MGI; MGI:94882; Defa2.
DR InParanoid; P28309; -.
DR PhylomeDB; P28309; -.
DR Reactome; R-MMU-1461973; Defensins.
DR Reactome; R-MMU-1462054; Alpha-defensins.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR PRO; PR:P28309; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P28309; protein.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IGI:MGI.
DR GO; GO:0042742; P:defense response to bacterium; IDA:MGI.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IGI:MGI.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IGI:MGI.
DR GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR GO; GO:0031640; P:killing of cells of another organism; ISO:MGI.
DR GO; GO:0051673; P:membrane disruption in another organism; IBA:GO_Central.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IDA:MGI.
DR InterPro; IPR016327; Alpha-defensin.
DR InterPro; IPR006081; Alpha-defensin_C.
DR InterPro; IPR002366; Alpha-defensin_propep.
DR InterPro; IPR006080; Defensin_beta/alpha.
DR PANTHER; PTHR11876; PTHR11876; 1.
DR Pfam; PF00323; Defensin_1; 1.
DR Pfam; PF00879; Defensin_propep; 1.
DR PIRSF; PIRSF001875; Alpha-defensin; 1.
DR SMART; SM00048; DEFSN; 1.
DR PROSITE; PS00269; DEFENSIN; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW Disulfide bond; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..58
FT /evidence="ECO:0000269|PubMed:1500163,
FT ECO:0000269|PubMed:1500431"
FT /id="PRO_0000006819"
FT PEPTIDE 59..93
FT /note="Alpha-defensin 2"
FT /id="PRO_0000006820"
FT REGION 23..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 64..92
FT /evidence="ECO:0000250"
FT DISULFID 66..81
FT /evidence="ECO:0000250"
FT DISULFID 71..91
FT /evidence="ECO:0000250"
FT CONFLICT 87
FT /note="M -> L (in Ref. 4; AAB60468)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 93 AA; 10560 MW; 4F05DEA252412B0D CRC64;
MKPLVLLSAL VLLSFQVQAD PIQNTDEETK TEEQSGEEDQ AVSVSFGDRE GASLQEESLR
DLVCYCRTRG CKRRERMNGT CRKGHLMYTL CCR
