DEFA3_MOUSE
ID DEFA3_MOUSE Reviewed; 93 AA.
AC P28310; Q499K7;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Alpha-defensin 3;
DE AltName: Full=Defensin-related cryptdin-3;
DE Flags: Precursor;
GN Name=Defa3; Synonyms=Defcr3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=129; TISSUE=Small intestine;
RX PubMed=8188287; DOI=10.1006/geno.1994.1093;
RA Huttner K.M., Selsted M.E., Ouellette A.J.;
RT "Structure and diversity of the murine cryptdin gene family.";
RL Genomics 19:448-453(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-93.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 59-93.
RC STRAIN=SWR/J; TISSUE=Small intestine;
RX PubMed=1500431; DOI=10.1083/jcb.118.4.929;
RA Selsted M.E., Miller S.I., Henschen A.H., Ouellette A.J.;
RT "Enteric defensins: antibiotic peptide components of intestinal host
RT defense.";
RL J. Cell Biol. 118:929-936(1992).
CC -!- FUNCTION: Probably contributes to the antimicrobial barrier function of
CC the small bowel mucosa.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Paneth cells of the small bowel.
CC -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
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DR EMBL; U12559; AAA20972.1; -; Genomic_DNA.
DR EMBL; U02999; AAB60677.1; -; Genomic_DNA.
DR EMBL; U02998; AAB60677.1; JOINED; Genomic_DNA.
DR EMBL; U03030; AAA57171.1; -; mRNA.
DR EMBL; BC099855; AAH99855.1; -; mRNA.
DR CCDS; CCDS40270.1; -.
DR PIR; I49102; I49102.
DR RefSeq; NP_001161262.1; NM_001167790.1.
DR RefSeq; NP_031876.1; NM_007850.2.
DR AlphaFoldDB; P28310; -.
DR SMR; P28310; -.
DR STRING; 10090.ENSMUSP00000078485; -.
DR iPTMnet; P28310; -.
DR PhosphoSitePlus; P28310; -.
DR PaxDb; P28310; -.
DR PeptideAtlas; P28310; -.
DR PRIDE; P28310; -.
DR DNASU; 13237; -.
DR Ensembl; ENSMUST00000079528; ENSMUSP00000078485; ENSMUSG00000060208.
DR Ensembl; ENSMUST00000098893; ENSMUSP00000096492; ENSMUSG00000074440.
DR GeneID; 13237; -.
DR GeneID; 23855; -.
DR KEGG; mmu:13237; -.
DR KEGG; mmu:23855; -.
DR UCSC; uc009lbn.2; mouse.
DR CTD; 1668; -.
DR CTD; 23855; -.
DR MGI; MGI:94883; Defa3.
DR VEuPathDB; HostDB:ENSMUSG00000060208; -.
DR VEuPathDB; HostDB:ENSMUSG00000074440; -.
DR eggNOG; ENOG502T2EX; Eukaryota.
DR GeneTree; ENSGT00940000153268; -.
DR HOGENOM; CLU_160803_1_0_1; -.
DR InParanoid; P28310; -.
DR OMA; CHLGERH; -.
DR OrthoDB; 1610714at2759; -.
DR PhylomeDB; P28310; -.
DR TreeFam; TF338414; -.
DR Reactome; R-MMU-1461973; Defensins.
DR Reactome; R-MMU-1462054; Alpha-defensins.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 13237; 4 hits in 10 CRISPR screens.
DR BioGRID-ORCS; 23855; 1 hit in 11 CRISPR screens.
DR PRO; PR:P28310; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; P28310; protein.
DR Bgee; ENSMUSG00000060208; Expressed in ileum and 15 other tissues.
DR GO; GO:0042582; C:azurophil granule; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IGI:MGI.
DR GO; GO:0042742; P:defense response to bacterium; IDA:MGI.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IGI:MGI.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IGI:MGI.
DR GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR GO; GO:0031640; P:killing of cells of another organism; ISO:MGI.
DR GO; GO:0051673; P:membrane disruption in another organism; IBA:GO_Central.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IDA:MGI.
DR InterPro; IPR016327; Alpha-defensin.
DR InterPro; IPR006081; Alpha-defensin_C.
DR InterPro; IPR002366; Alpha-defensin_propep.
DR InterPro; IPR006080; Defensin_beta/alpha.
DR PANTHER; PTHR11876; PTHR11876; 1.
DR Pfam; PF00323; Defensin_1; 1.
DR Pfam; PF00879; Defensin_propep; 1.
DR PIRSF; PIRSF001875; Alpha-defensin; 1.
DR SMART; SM00048; DEFSN; 1.
DR PROSITE; PS00269; DEFENSIN; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW Disulfide bond; Reference proteome; Secreted; Signal.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT PROPEP 17..58
FT /evidence="ECO:0000269|PubMed:1500431"
FT /id="PRO_0000006821"
FT PEPTIDE 59..93
FT /note="Alpha-defensin 3"
FT /id="PRO_0000006822"
FT REGION 22..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 64..92
FT /evidence="ECO:0000250"
FT DISULFID 66..81
FT /evidence="ECO:0000250"
FT DISULFID 71..91
FT /evidence="ECO:0000250"
SQ SEQUENCE 93 AA; 10528 MW; 2B2D61CFCF40E634 CRC64;
MKTLVLLSAL VLLAFQVQAD PIQNTDEETK TEEQPGEDDQ AVSVSFGDPE GSSLQEESLR
DLVCYCRKRG CKRRERMNGT CRKGHLMYTL CCR
