DEFA5_MOUSE
ID DEFA5_MOUSE Reviewed; 93 AA.
AC P28312; Q64382;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Alpha-defensin 5;
DE AltName: Full=Defensin-related cryptdin-5;
DE Flags: Precursor;
GN Name=Defa5; Synonyms=Defcr5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129, and C3H/HeJ; TISSUE=Intestinal epithelium;
RX PubMed=8188287; DOI=10.1006/geno.1994.1093;
RA Huttner K.M., Selsted M.E., Ouellette A.J.;
RT "Structure and diversity of the murine cryptdin gene family.";
RL Genomics 19:448-453(1994).
RN [2]
RP PROTEIN SEQUENCE OF 59-93.
RC STRAIN=SWR/J; TISSUE=Small intestine;
RX PubMed=1500431; DOI=10.1083/jcb.118.4.929;
RA Selsted M.E., Miller S.I., Henschen A.H., Ouellette A.J.;
RT "Enteric defensins: antibiotic peptide components of intestinal host
RT defense.";
RL J. Cell Biol. 118:929-936(1992).
CC -!- FUNCTION: Probably contributes to the antimicrobial barrier function of
CC the small bowel mucosa.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
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DR EMBL; U12560; AAA20973.1; -; Genomic_DNA.
DR EMBL; U03001; AAB60678.1; -; Genomic_DNA.
DR EMBL; U03000; AAB60678.1; JOINED; Genomic_DNA.
DR EMBL; U03033; AAA57173.1; -; mRNA.
DR CCDS; CCDS22152.1; -.
DR PIR; I49103; I49103.
DR AlphaFoldDB; P28312; -.
DR SMR; P28312; -.
DR STRING; 10090.ENSMUSP00000077259; -.
DR PaxDb; P28312; -.
DR PRIDE; P28312; -.
DR MGI; MGI:99583; Defa5.
DR InParanoid; P28312; -.
DR Reactome; R-MMU-1461973; Defensins.
DR Reactome; R-MMU-1462054; Alpha-defensins.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR PRO; PR:P28312; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P28312; protein.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0030496; C:midbody; ISO:MGI.
DR GO; GO:0030141; C:secretory granule; ISO:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; ISO:MGI.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IDA:MGI.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; ISO:MGI.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; ISO:MGI.
DR GO; GO:0045087; P:innate immune response; ISO:MGI.
DR GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR GO; GO:0051873; P:killing by host of symbiont cells; ISO:MGI.
DR GO; GO:0031640; P:killing of cells of another organism; ISO:MGI.
DR GO; GO:0051673; P:membrane disruption in another organism; ISO:MGI.
DR GO; GO:0032757; P:positive regulation of interleukin-8 production; ISO:MGI.
DR GO; GO:1905710; P:positive regulation of membrane permeability; ISO:MGI.
DR GO; GO:0051289; P:protein homotetramerization; ISO:MGI.
DR InterPro; IPR016327; Alpha-defensin.
DR InterPro; IPR006081; Alpha-defensin_C.
DR InterPro; IPR002366; Alpha-defensin_propep.
DR InterPro; IPR006080; Defensin_beta/alpha.
DR PANTHER; PTHR11876; PTHR11876; 1.
DR Pfam; PF00323; Defensin_1; 1.
DR Pfam; PF00879; Defensin_propep; 1.
DR PIRSF; PIRSF001875; Alpha-defensin; 1.
DR SMART; SM00048; DEFSN; 1.
DR PROSITE; PS00269; DEFENSIN; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW Disulfide bond; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..58
FT /evidence="ECO:0000269|PubMed:1500431"
FT /id="PRO_0000006825"
FT PEPTIDE 59..93
FT /note="Alpha-defensin 5"
FT /id="PRO_0000006826"
FT DISULFID 64..92
FT /evidence="ECO:0000250"
FT DISULFID 66..81
FT /evidence="ECO:0000250"
FT DISULFID 71..91
FT /evidence="ECO:0000250"
FT CONFLICT 59
FT /note="S -> SA (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 93 AA; 10519 MW; 7FC27CBBEFB6259E CRC64;
MKTFVLLSAL VLLAFQVQAD PIHKTDEETN TEEQPGEEDQ AVSISFGGQE GSALHEELSK
KLICYCRIRG CKRRERVFGT CRNLFLTFVF CCS
