DEFA6_MOUSE
ID DEFA6_MOUSE Reviewed; 93 AA.
AC P50704; P50710; P82294;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Alpha-defensin 6/12;
DE AltName: Full=Defensin-related cryptdin-6/12;
DE Short=Cryptdin-2;
DE Flags: Precursor;
GN Name=Defa6; Synonyms=Defcr6;
GN and
GN Name=Defa12; Synonyms=Defcr12;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (DEFA6), AND NUCLEOTIDE SEQUENCE
RP [MRNA] OF 59-93 (DEFCR12).
RC STRAIN=129, and C3H/HeJ; TISSUE=Intestinal epithelium;
RX PubMed=8188287; DOI=10.1006/geno.1994.1093;
RA Huttner K.M., Selsted M.E., Ouellette A.J.;
RT "Structure and diversity of the murine cryptdin gene family.";
RL Genomics 19:448-453(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (DEFA6 AND DEFA12), AND FUNCTION.
RC STRAIN=CD-1; TISSUE=Intestinal crypt;
RX PubMed=7927786; DOI=10.1128/iai.62.11.5040-5047.1994;
RA Ouellette A.J., Hsieh M.M., Nosek M.T., Cano-Gauci D.F., Huttner K.M.,
RA Buick R.N., Selsted M.E.;
RT "Mouse Paneth cell defensins: primary structures and antibacterial
RT activities of numerous cryptdin isoforms.";
RL Infect. Immun. 62:5040-5047(1994).
RN [3]
RP PROTEIN SEQUENCE OF 59-93, AND FUNCTION.
RC STRAIN=Swiss Webster; TISSUE=Small intestine;
RX PubMed=1500163; DOI=10.1128/iai.60.9.3556-3565.1992;
RA Eisenhauer P.B., Harwig S.S.S.L., Lehrer R.I.;
RT "Cryptdins: antimicrobial defensins of the murine small intestine.";
RL Infect. Immun. 60:3556-3565(1992).
CC -!- FUNCTION: Has broad-spectrum antimicrobial properties. Has
CC antibacterial activity against the Gram-positive bacterium
CC L.monocytogenes EGD and the Gram-negative bacteria E.coli ML-35p and
CC avirulent S.typhimurium 7953, but not against the mouse-virulent
CC S.typhimurium 14028S. Probably contributes to the antimicrobial barrier
CC function of the small bowel mucosa. {ECO:0000269|PubMed:1500163,
CC ECO:0000269|PubMed:7927786}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Paneth cells of the small bowel.
CC -!- SIMILARITY: Belongs to the alpha-defensin family. {ECO:0000305}.
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DR EMBL; U03003; AAB60679.1; -; Genomic_DNA.
DR EMBL; U03002; AAB60679.1; JOINED; Genomic_DNA.
DR EMBL; U03034; AAA57174.1; -; mRNA.
DR EMBL; U03063; AAA57180.1; -; mRNA.
DR EMBL; U12561; AAA20974.1; -; Genomic_DNA.
DR PIR; I49104; I49104.
DR RefSeq; NP_031878.1; NM_007852.1.
DR AlphaFoldDB; P50704; -.
DR SMR; P50704; -.
DR PhosphoSitePlus; P50704; -.
DR MaxQB; P50704; -.
DR PeptideAtlas; P50704; -.
DR PRIDE; P50704; -.
DR DNASU; 13240; -.
DR GeneID; 13240; -.
DR KEGG; mmu:13240; -.
DR UCSC; uc009lbx.2; mouse.
DR CTD; 1671; -.
DR MGI; MGI:99589; Defa12.
DR MGI; MGI:99582; Defa6.
DR InParanoid; P50704; -.
DR BioGRID-ORCS; 13240; 3 hits in 15 CRISPR screens.
DR PRO; PR:P50704; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P50704; protein.
DR GO; GO:0005615; C:extracellular space; IDA:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0030496; C:midbody; ISO:MGI.
DR GO; GO:0030141; C:secretory granule; ISO:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:MGI.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; ISO:MGI.
DR GO; GO:0071222; P:cellular response to lipopolysaccharide; IGI:MGI.
DR GO; GO:0042742; P:defense response to bacterium; IDA:MGI.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IGI:MGI.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IGI:MGI.
DR GO; GO:0045087; P:innate immune response; ISO:MGI.
DR GO; GO:0002227; P:innate immune response in mucosa; IBA:GO_Central.
DR GO; GO:0051873; P:killing by host of symbiont cells; ISO:MGI.
DR GO; GO:0031640; P:killing of cells of another organism; ISO:MGI.
DR GO; GO:0051673; P:membrane disruption in another organism; ISO:MGI.
DR GO; GO:0032757; P:positive regulation of interleukin-8 production; ISO:MGI.
DR GO; GO:1905710; P:positive regulation of membrane permeability; ISO:MGI.
DR GO; GO:0051289; P:protein homotetramerization; ISO:MGI.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IDA:MGI.
DR InterPro; IPR016327; Alpha-defensin.
DR InterPro; IPR006081; Alpha-defensin_C.
DR InterPro; IPR002366; Alpha-defensin_propep.
DR InterPro; IPR006080; Defensin_beta/alpha.
DR PANTHER; PTHR11876; PTHR11876; 1.
DR Pfam; PF00323; Defensin_1; 1.
DR Pfam; PF00879; Defensin_propep; 1.
DR PIRSF; PIRSF001875; Alpha-defensin; 1.
DR SMART; SM00048; DEFSN; 1.
DR PROSITE; PS00269; DEFENSIN; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW Disulfide bond; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..60
FT /evidence="ECO:0000250"
FT /id="PRO_0000006827"
FT PEPTIDE 61..93
FT /note="Alpha-defensin 6/12"
FT /id="PRO_0000006828"
FT REGION 23..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 64..92
FT /evidence="ECO:0000250"
FT DISULFID 66..81
FT /evidence="ECO:0000250"
FT DISULFID 71..91
FT /evidence="ECO:0000250"
FT VARIANT 61
FT /note="D -> Y (in strain: C3H/HeJ)"
FT VARIANT 87
FT /note="L -> M (in strain: C3H/HeJ)"
FT VARIANT 89
FT /note="M -> T (in strain: C3H/HeJ)"
SQ SEQUENCE 93 AA; 10426 MW; 84CE684E476FB885 CRC64;
MKTLILLSAL VLLAFQVQAD PIQNTDEETK TEEQPGEEDQ AVSVSFGDPE GTSLQEESLR
DLVCYCRARG CKGRERMNGT CRKGHLLYML CCR
