ID   DEFA_ANOCP              Reviewed;          43 AA.
AC   P83669;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Defensin-A;
OS   Anomala cuprea (Cupreous chafer beetle).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Coleoptera; Polyphaga; Scarabaeiformia;
OC   Scarabaeidae; Rutelinae; Anomala.
OX   NCBI_TaxID=121601 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Larval hemolymph {ECO:0000269|PubMed:11719071};
RX   PubMed=11719071; DOI=10.1016/s0965-1748(01)00082-0;
RA   Yamauchi H.;
RT   "Two novel insect defensins from larvae of the cupreous chafer, Anomala
RT   cuprea: purification, amino acid sequences and antibacterial activity.";
RL   Insect Biochem. Mol. Biol. 32:75-84(2001).
CC   -!- FUNCTION: Antibacterial protein. Strong activity against the Gram-
CC       positive bacteria M.luteus, B.megaterium and S.aureus. Reduced activity
CC       against Gram-positive bacterium B.subtilis and weak activity against
CC       Gram-negative bacterium X.japonicus. No detectable activity against the
CC       Gram-negative bacteria E.asbriae, E.coli, P.aeruginosa and
CC       S.marcescens. {ECO:0000269|PubMed:11719071}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00710,
CC       ECO:0000269|PubMed:11719071}.
CC   -!- MASS SPECTROMETRY: Mass=4421.6; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:11719071};
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 1
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR   AlphaFoldDB; P83669; -.
DR   SMR; P83669; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR017982; Defensin_insect.
DR   InterPro; IPR001542; Defensin_invertebrate/fungal.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   Pfam; PF01097; Defensin_2; 1.
DR   PRINTS; PR00271; DEFENSIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Immunity; Innate immunity; Secreted.
FT   CHAIN           1..43
FT                   /note="Defensin-A"
FT                   /id="PRO_0000074469"
FT   DISULFID        3..34
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT   DISULFID        20..39
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT   DISULFID        24..41
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
SQ   SEQUENCE   43 AA;  4426 MW;  3C53717C0E780451 CRC64;
     VTCDLLSFEA KGFAANHSIC AAHCLAIGRK GGSCQNGVCV CRN