ID   DEFB4_BOVIN             Reviewed;          63 AA.
AC   P46162;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 3.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Beta-defensin 4;
DE   AltName: Full=BNBD-4;
DE   AltName: Full=BNDB-4;
DE   Flags: Precursor;
GN   Name=DEFB4; Synonyms=BNBD4;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Yount N.Y., Yuan J., Diamond G., Tarver A., McGuire P.A., McCullough C.,
RA   Cullor J.S., Bevins C.L., Selsted M.E.;
RT   "Molecular cloning and expression of an antimicrobial beta-defensin from
RT   bovine neutrophils. Characterization of BNBD-4 cDNA and genomic sequences
RT   and localization of the peptide to large granules of mature neutrophils.";
RL   Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RA   Yount N.Y., Yuan J., Tarver A.P., Diamond G., Levy J.N., McGuire P.A.,
RA   McCullough C., Cullor J.S., Bevins C.L., Selsted M.E.;
RL   Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RA   Yount N.Y., Yuan J., Tarver A.P., Diamond G., Levy J.N., McGuire P.A.,
RA   McCullough C., Cullor J.S., Bevins C.L., Selsted M.E.;
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE.
RA   Ryan L.K., Rhodes J., Bhat M., Diamond G.;
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   PROTEIN SEQUENCE OF 23-63, AND PYROGLUTAMATE FORMATION AT GLN-23.
RC   STRAIN=Hereford; TISSUE=Neutrophil;
RX   PubMed=8454635; DOI=10.1016/s0021-9258(18)53298-1;
RA   Selsted M.E., Tang Y.-Q., Morris W.L., McGuire P.A., Novotny M.J.,
RA   Smith W., Henschen A.H., Cullor J.S.;
RT   "Purification, primary structures, and antibacterial activities of beta-
RT   defensins, a new family of antimicrobial peptides from bovine
RT   neutrophils.";
RL   J. Biol. Chem. 268:6641-6648(1993).
CC   -!- FUNCTION: Has bactericidal activity. Active against E.coli ML35 and
CC       S.aureus 502A.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Neutrophilic granules.
CC   -!- SIMILARITY: Belongs to the beta-defensin family. {ECO:0000305}.
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DR   EMBL; U36200; AAD10283.1; -; mRNA.
DR   EMBL; AF008307; AAB63292.1; -; Genomic_DNA.
DR   EMBL; AF014107; AAD01522.1; -; mRNA.
DR   PIR; D45495; D45495.
DR   RefSeq; NP_777200.1; NM_174775.1.
DR   AlphaFoldDB; P46162; -.
DR   SMR; P46162; -.
DR   STRING; 9913.ENSBTAP00000055285; -.
DR   PaxDb; P46162; -.
DR   PRIDE; P46162; -.
DR   Ensembl; ENSBTAT00000082114; ENSBTAP00000066174; ENSBTAG00000053557.
DR   GeneID; 286836; -.
DR   KEGG; bta:286836; -.
DR   CTD; 1673; -.
DR   VEuPathDB; HostDB:ENSBTAG00000053557; -.
DR   GeneTree; ENSGT00940000160995; -.
DR   InParanoid; P46162; -.
DR   OMA; CIPFLCR; -.
DR   Proteomes; UP000009136; Chromosome 27.
DR   Bgee; ENSBTAG00000053557; Expressed in pharyngeal tonsil and 76 other tissues.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0031731; F:CCR6 chemokine receptor binding; IBA:GO_Central.
DR   GO; GO:0042056; F:chemoattractant activity; IBA:GO_Central.
DR   GO; GO:0060326; P:cell chemotaxis; IBA:GO_Central.
DR   GO; GO:0042742; P:defense response to bacterium; IBA:GO_Central.
DR   InterPro; IPR001855; Defensin_beta-typ.
DR   InterPro; IPR006080; Defensin_beta/alpha.
DR   Pfam; PF00711; Defensin_beta; 1.
DR   SMART; SM00048; DEFSN; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Pyrrolidone carboxylic acid; Reference proteome; Secreted;
KW   Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000269|PubMed:8454635"
FT   PEPTIDE         23..63
FT                   /note="Beta-defensin 4"
FT                   /id="PRO_0000006882"
FT   MOD_RES         23
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:8454635"
FT   DISULFID        31..60
FT                   /evidence="ECO:0000250"
FT   DISULFID        38..53
FT                   /evidence="ECO:0000250"
FT   DISULFID        43..61
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   63 AA;  7161 MW;  3A4427EF57D654A3 CRC64;
     MRLHHLLLAV LFLVLSAGSG FTQRVRNPQS CRWNMGVCIP FLCRVGMRQI GTCFGPRVPC
     CRR