DEFB_AEDAE
ID DEFB_AEDAE Reviewed; 98 AA.
AC P81602; Q71U14; Q71U15;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Defensin-B;
DE Flags: Precursor;
GN Name=DEFB; ORFNames=AAEL003857;
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=Liverpool; TISSUE=Fat body;
RX PubMed=9927179; DOI=10.1046/j.1365-2583.1999.810107.x;
RA Lowenberger C.A., Smartt C.T., Bulet P., Ferdig M.T., Severson D.W.,
RA Hoffmann J.A., Christensen B.M.;
RT "Insect immunity: molecular cloning, expression, and characterization of
RT cDNAs and genomic DNA encoding three isoforms of insect defensin in Aedes
RT aegypti.";
RL Insect Mol. Biol. 8:107-118(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVPib12;
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
RN [3]
RP PROTEIN SEQUENCE OF 59-98, FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=Liverpool;
RX PubMed=7633471; DOI=10.1016/0965-1748(95)00043-u;
RA Lowenberger C., Bulet P., Charlet M., Hetru C., Hodgeman B.,
RA Christensen B.M., Hoffmann J.A.;
RT "Insect immunity: isolation of three novel inducible antibacterial
RT defensins from the vector mosquito, Aedes aegypti.";
RL Insect Biochem. Mol. Biol. 25:867-873(1995).
CC -!- FUNCTION: Antibacterial peptide mostly active against Gram-positive
CC bacteria. {ECO:0000255|PROSITE-ProRule:PRU00710,
CC ECO:0000269|PubMed:7633471}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00710,
CC ECO:0000269|PubMed:7633471}.
CC -!- INDUCTION: By bacterial infection. {ECO:0000269|PubMed:7633471}.
CC -!- POLYMORPHISM: There are two defensin B isoforms, B1 (shown here) and
CC B2.
CC -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 1
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR EMBL; AF156090; AAD40114.2; -; mRNA.
DR EMBL; AF156091; AAD40115.2; -; mRNA.
DR EMBL; CH477283; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; P81602; -.
DR VEuPathDB; VectorBase:AAEL003841; -.
DR HOGENOM; CLU_174272_0_0_1; -.
DR InParanoid; P81602; -.
DR OrthoDB; 1600872at2759; -.
DR Proteomes; UP000008820; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:UniProt.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR001542; Defensin_invertebrate/fungal.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR Pfam; PF01097; Defensin_2; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW Disulfide bond; Immunity; Innate immunity; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT PROPEP 21..58
FT /evidence="ECO:0000269|PubMed:7633471"
FT /id="PRO_0000289969"
FT PEPTIDE 59..98
FT /note="Defensin-B"
FT /id="PRO_0000044708"
FT DISULFID 61..88
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT DISULFID 74..94
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT DISULFID 78..96
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT VARIANT 15..16
FT /note="VA -> GS (in isoform B2)"
SQ SEQUENCE 98 AA; 10583 MW; 17F9457B90E66843 CRC64;
MKSITVICFL ALCTVAITSA YPQEPVLADE ARPFANSLFD ELPEETYQAA VENFRLKRAT
CDLLSGFGVG DSACAAHCIA RGNRGGYCNS QKVCVCRN
