DEFC_AEDAE
ID DEFC_AEDAE Reviewed; 99 AA.
AC P81603; Q17EE4; Q9Y0F0; Q9Y0F1;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 3.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Defensin-C;
DE Flags: Precursor;
GN Name=DEFC; ORFNames=AAEL003832;
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, INDUCTION, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=Liverpool; TISSUE=Fat body;
RX PubMed=9927179; DOI=10.1046/j.1365-2583.1999.810107.x;
RA Lowenberger C.A., Smartt C.T., Bulet P., Ferdig M.T., Severson D.W.,
RA Hoffmann J.A., Christensen B.M.;
RT "Insect immunity: molecular cloning, expression, and characterization of
RT cDNAs and genomic DNA encoding three isoforms of insect defensin in Aedes
RT aegypti.";
RL Insect Mol. Biol. 8:107-118(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVPib12;
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
RN [3]
RP PROTEIN SEQUENCE OF 60-99, AND INDUCTION.
RC STRAIN=Liverpool;
RX PubMed=7633471; DOI=10.1016/0965-1748(95)00043-u;
RA Lowenberger C., Bulet P., Charlet M., Hetru C., Hodgeman B.,
RA Christensen B.M., Hoffmann J.A.;
RT "Insect immunity: isolation of three novel inducible antibacterial
RT defensins from the vector mosquito, Aedes aegypti.";
RL Insect Biochem. Mol. Biol. 25:867-873(1995).
RN [4]
RP PROTEIN SEQUENCE OF 60-99.
RC STRAIN=REFM;
RX PubMed=7568275; DOI=10.1098/rspb.1995.0139;
RA Chalk R., Albuquerque C.M., Ham P.J., Townson H.;
RT "Full sequence and characterization of two insect defensins: immune
RT peptides from the mosquito Aedes aegypti.";
RL Proc. R. Soc. B 261:217-221(1995).
CC -!- FUNCTION: Antibacterial peptide mostly active against Gram-positive
CC bacteria.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00710,
CC ECO:0000269|PubMed:9927179}.
CC -!- TISSUE SPECIFICITY: Hemolymph.
CC -!- DEVELOPMENTAL STAGE: Expressed 30 minutes after infection and remained
CC present through to 21 days. Expressed in white or callow pupae during
CC metamorphosis, but no expression was seen in larvae.
CC {ECO:0000269|PubMed:9927179}.
CC -!- INDUCTION: By bacterial infection. {ECO:0000269|PubMed:7633471,
CC ECO:0000269|PubMed:9927179}.
CC -!- POLYMORPHISM: There are two defensin C isoforms, C1 and C2 (shown
CC here).
CC -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 1
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR EMBL; AF156092; AAD40116.2; -; mRNA.
DR EMBL; AF156093; AAD40117.2; -; mRNA.
DR EMBL; CH477283; EAT44832.1; -; Genomic_DNA.
DR RefSeq; XP_001657288.1; XM_001657238.2.
DR AlphaFoldDB; P81603; -.
DR STRING; 7159.AAEL003832-PA; -.
DR GeneID; 5579094; -.
DR KEGG; aag:5579094; -.
DR VEuPathDB; VectorBase:AAEL003832; -.
DR eggNOG; ENOG502SD3P; Eukaryota.
DR HOGENOM; CLU_174272_0_0_1; -.
DR InParanoid; P81603; -.
DR OrthoDB; 1600872at2759; -.
DR Proteomes; UP000008820; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:UniProt.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR001542; Defensin_invertebrate/fungal.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR Pfam; PF01097; Defensin_2; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Cleavage on pair of basic residues; Defensin;
KW Direct protein sequencing; Disulfide bond; Immunity; Innate immunity;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..59
FT /evidence="ECO:0000305"
FT /id="PRO_0000006736"
FT CHAIN 60..99
FT /note="Defensin-C"
FT /evidence="ECO:0000269|PubMed:7568275,
FT ECO:0000269|PubMed:7633471"
FT /id="PRO_0000006737"
FT DISULFID 62..89
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT DISULFID 75..95
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT DISULFID 79..97
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT VARIANT 26
FT /note="G -> E (in isoform C1)"
FT VARIANT 34
FT /note="P -> S (in isoform C1)"
FT CONFLICT 5
FT /note="T -> I (in Ref. 1; AAD40116/AAD40117)"
FT /evidence="ECO:0000305"
FT CONFLICT 22
FT /note="N -> S (in Ref. 1; AAD40116/AAD40117)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="R -> G (in Ref. 3; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 91
FT /note="A -> S (in Ref. 3; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 99 AA; 10711 MW; 7914827671AF6709 CRC64;
MRTLTVVCFV ALCLSAIFTT GNALPGELAD DVRPYANSLF DELPEESYQA AVENFRLKRA
TCDLLSGFGV GDSACAAHCI ARRNRGGYCN AKKVCVCRN
