ID   DEFEU_ASPOR             Reviewed;          69 AA.
AC   Q2U3M0;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   22-APR-2020, sequence version 2.
DT   25-MAY-2022, entry version 50.
DE   RecName: Full=Fungal defensin oryzeasin {ECO:0000303|PubMed:20508130};
DE   Flags: Precursor;
GN   ORFNames=AO090020000702;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
RN   [2]
RP   FUNCTION, AND BINDING TO LIPID II.
RX   PubMed=20508130; DOI=10.1126/science.1185723;
RA   Schneider T., Kruse T., Wimmer R., Wiedemann I., Sass V., Pag U.,
RA   Jansen A., Nielsen A.K., Mygind P.H., Raventos D.S., Neve S., Ravn B.,
RA   Bonvin A.M., De Maria L., Andersen A.S., Gammelgaard L.K., Sahl H.G.,
RA   Kristensen H.H.;
RT   "Plectasin, a fungal defensin, targets the bacterial cell wall precursor
RT   Lipid II.";
RL   Science 328:1168-1172(2010).
CC   -!- FUNCTION: Shows antibacterial activity against numerous Gram-positive
CC       bacteria (Probable). It selectively inhibits peptidoglycan biosynthesis
CC       through complex formation with the cell wall precursor lipid II (1:1
CC       molar ratio) thus inhibiting cell wall synthesis (PubMed:20508130).
CC       {ECO:0000305|PubMed:20508130}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Target cell membrane
CC       {ECO:0000305|PubMed:20508130}.
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE63845.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305|PubMed:20508130};
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DR   EMBL; AP007167; BAE63845.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_001824978.2; XM_001824926.2.
DR   AlphaFoldDB; Q2U3M0; -.
DR   EnsemblFungi; BAE63845; BAE63845; AO090020000702.
DR   HOGENOM; CLU_2305451_0_0_1; -.
DR   Proteomes; UP000006564; Chromosome 6.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0002376; P:immune system process; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues; Defensin;
KW   Disulfide bond; Immunity; Innate immunity; Lipid-binding; Membrane;
KW   Reference proteome; Secreted; Signal; Target cell membrane;
KW   Target membrane.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   PROPEP          19..26
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_0000449382"
FT   CHAIN           27..69
FT                   /note="Fungal defensin oryzeasin"
FT                   /id="PRO_5004216947"
FT   DISULFID        29..60
FT                   /evidence="ECO:0000305"
FT   DISULFID        44..66
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT   DISULFID        48..68
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
SQ   SEQUENCE   69 AA;  7278 MW;  C8B8BD6175818CA7 CRC64;
     MKLLTVAFSL LLLGQVHASP LVLDKRSSCQ LGDVWDLNAA DAACSASCAI QHGDKHGGHC
     DKNKVCVCN