ID   DEFI8_BOVIN             Reviewed;         447 AA.
AC   A5PJM7;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Differentially expressed in FDCP 8 homolog;
DE            Short=DEF-8;
GN   Name=DEF8;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Hippocampus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Positively regulates lysosome peripheral distribution and
CC       ruffled border formation in osteoclasts. Involved in bone resorption.
CC       {ECO:0000250|UniProtKB:Q99J78}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with PLEKHM1; this interaction is
CC       weak but increased in a RAB7A-dependent manner.
CC       {ECO:0000250|UniProtKB:Q99J78}.
CC   -!- SIMILARITY: Belongs to the DEF8 family. {ECO:0000305}.
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DR   EMBL; BC142174; AAI42175.1; -; mRNA.
DR   RefSeq; NP_001092427.1; NM_001098957.1.
DR   AlphaFoldDB; A5PJM7; -.
DR   SMR; A5PJM7; -.
DR   STRING; 9913.ENSBTAP00000004669; -.
DR   PaxDb; A5PJM7; -.
DR   PRIDE; A5PJM7; -.
DR   Ensembl; ENSBTAT00000004669; ENSBTAP00000004669; ENSBTAG00000039014.
DR   GeneID; 513349; -.
DR   KEGG; bta:513349; -.
DR   CTD; 54849; -.
DR   VEuPathDB; HostDB:ENSBTAG00000039014; -.
DR   VGNC; VGNC:27987; DEF8.
DR   eggNOG; KOG1829; Eukaryota.
DR   GeneTree; ENSGT00940000159182; -.
DR   InParanoid; A5PJM7; -.
DR   OrthoDB; 177737at2759; -.
DR   Proteomes; UP000009136; Chromosome 18.
DR   Bgee; ENSBTAG00000039014; Expressed in neutrophil and 103 other tissues.
DR   ExpressionAtlas; A5PJM7; baseline and differential.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0032418; P:lysosome localization; ISS:UniProtKB.
DR   GO; GO:0045780; P:positive regulation of bone resorption; ISS:UniProtKB.
DR   GO; GO:1900029; P:positive regulation of ruffle assembly; ISS:UniProtKB.
DR   InterPro; IPR046349; C1-like_sf.
DR   InterPro; IPR002219; PE/DAG-bd.
DR   InterPro; IPR025258; Zf-RING_9.
DR   Pfam; PF00130; C1_1; 1.
DR   Pfam; PF13901; zf-RING_9; 1.
DR   SMART; SM00109; C1; 2.
DR   SMART; SM01175; DUF4206; 1.
DR   SUPFAM; SSF57889; SSF57889; 1.
DR   PROSITE; PS00479; ZF_DAG_PE_1; 1.
DR   PROSITE; PS50081; ZF_DAG_PE_2; 1.
PE   2: Evidence at transcript level;
KW   Metal-binding; Phosphoprotein; Reference proteome; Repeat; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..447
FT                   /note="Differentially expressed in FDCP 8 homolog"
FT                   /id="PRO_0000321912"
FT   ZN_FING         134..185
FT                   /note="Phorbol-ester/DAG-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00226"
FT   ZN_FING         364..424
FT                   /note="Phorbol-ester/DAG-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00226"
FT   REGION          17..47
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         436
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q99J78"
SQ   SEQUENCE   447 AA;  51847 MW;  55976B4CAD440069 CRC64;
     MEYDEKLARF RQVHLNPFNK QQHEQGAGEE APGITSQARL PELPPGEPEF RCPERVMDLG
     LSEDHFARPV GLFLASDVQQ LRQAIQECKQ VILDLPEHSE KQKDAVVRLI HLRLKLQELK
     DPNEDEPNIR VLLEHRFYKE KSKSVKQTCD KCNTVIWGLI QTWYTCTGCY YRCHSKCLNL
     ISKPCVRSKV SHQAEYELNI CPEAGLDSQD YRCAECRAPI SLRGVPSEAR QCDYTGQYYC
     SHCHWNDLAV IPARVVHNWD FEPRKVSRGS MRYLALMMSR PVLRLREINP LLFNYVEELV
     EIRKLRQDIL LMKPYFITCR EAMAARLLLQ LQDRQHFVEN DEMYSVQDLL DTHTGRLGCS
     LAETHTLFAK HIKLDCERCQ AKGFVCELCR EGDVLFPFDS HTSVCADCSA VFHRDCYYDN
     STTCPRCARL TLRKQSLFRE PGPDLDA