ID   DEFI8_RAT               Reviewed;         451 AA.
AC   Q4V8I4;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Differentially expressed in FDCP 8 homolog;
DE            Short=DEF-8;
GN   Name=Def8;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Placenta;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-440, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: Positively regulates lysosome peripheral distribution and
CC       ruffled border formation in osteoclasts. Involved in bone resorption.
CC       {ECO:0000250|UniProtKB:Q99J78}.
CC   -!- SUBUNIT: Interacts (via C-terminus) with PLEKHM1; this interaction is
CC       weak but increased in a RAB7A-dependent manner.
CC       {ECO:0000250|UniProtKB:Q99J78}.
CC   -!- SIMILARITY: Belongs to the DEF8 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC097376; AAH97376.1; -; mRNA.
DR   RefSeq; NP_001019945.1; NM_001024774.1.
DR   RefSeq; XP_006255831.1; XM_006255769.2.
DR   AlphaFoldDB; Q4V8I4; -.
DR   SMR; Q4V8I4; -.
DR   STRING; 10116.ENSRNOP00000000281; -.
DR   iPTMnet; Q4V8I4; -.
DR   PhosphoSitePlus; Q4V8I4; -.
DR   PaxDb; Q4V8I4; -.
DR   PRIDE; Q4V8I4; -.
DR   Ensembl; ENSRNOT00000000281; ENSRNOP00000000281; ENSRNOG00000000264.
DR   GeneID; 307973; -.
DR   KEGG; rno:307973; -.
DR   UCSC; RGD:1309008; rat.
DR   CTD; 54849; -.
DR   RGD; 1309008; Def8.
DR   eggNOG; KOG1829; Eukaryota.
DR   GeneTree; ENSGT00940000159182; -.
DR   HOGENOM; CLU_034500_4_0_1; -.
DR   InParanoid; Q4V8I4; -.
DR   OMA; IEPRLCD; -.
DR   OrthoDB; 177737at2759; -.
DR   PhylomeDB; Q4V8I4; -.
DR   TreeFam; TF317067; -.
DR   PRO; PR:Q4V8I4; -.
DR   Proteomes; UP000002494; Chromosome 19.
DR   Bgee; ENSRNOG00000000264; Expressed in adult mammalian kidney and 18 other tissues.
DR   Genevisible; Q4V8I4; RN.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0032418; P:lysosome localization; ISS:UniProtKB.
DR   GO; GO:0045780; P:positive regulation of bone resorption; ISS:UniProtKB.
DR   GO; GO:1900029; P:positive regulation of ruffle assembly; ISS:UniProtKB.
DR   InterPro; IPR046349; C1-like_sf.
DR   InterPro; IPR002219; PE/DAG-bd.
DR   InterPro; IPR025258; Zf-RING_9.
DR   Pfam; PF00130; C1_1; 1.
DR   Pfam; PF13901; zf-RING_9; 1.
DR   SMART; SM00109; C1; 2.
DR   SMART; SM01175; DUF4206; 1.
DR   SUPFAM; SSF57889; SSF57889; 1.
DR   PROSITE; PS00479; ZF_DAG_PE_1; 1.
DR   PROSITE; PS50081; ZF_DAG_PE_2; 1.
PE   1: Evidence at protein level;
KW   Metal-binding; Phosphoprotein; Reference proteome; Repeat; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..451
FT                   /note="Differentially expressed in FDCP 8 homolog"
FT                   /id="PRO_0000321915"
FT   ZN_FING         138..189
FT                   /note="Phorbol-ester/DAG-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00226"
FT   ZN_FING         368..428
FT                   /note="Phorbol-ester/DAG-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00226"
FT   REGION          18..48
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        21..40
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         440
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
SQ   SEQUENCE   451 AA;  52697 MW;  F98099AF3B30D2D2 CRC64;
     MEYDEKLVRF RQAHLNPFNK HLGPRHHEQE PNEKAQEVTS EDTLPELPAG EPEFCYSERM
     MDLGLSEDHF SRPVGLFLAS DVQQLRQAIE ECKQVILELP EQSEKQKDAV VRLIHLRLKL
     QELKDPNEEE PNIRVLLEHR FYKEKSKSVK QTCDKCNTII WGLIQTWYTC TGCYYRCHSK
     CLNLISRPCV SSKVSHQAEY ELNICPETGL DSQDYRCAEC RAPISLRGVP SEARQCDYTG
     QYYCSHCHWN DLAVIPARVV HNWDFEPRKV SRCSMRYLAL MVSRPVLRLR EINPLLFNYV
     EELVEIRKLR QDILLMKPYF ITCKEAMEAR LLLQLQDRQH FVENDEMYSI QDLLEVHMGR
     LSCSLTEIHT IFAKHIKLDC ERCQAKGFVC ELCKEGDVLF PFDSHTSVCN DCSAVFHRDC
     YYDNSTTCPK CARLTLRKQS LFQEPGLDVD A