ID   DEFI_APICA              Reviewed;          95 AA.
AC   Q5J8R1;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Defensin-1;
DE   Flags: Precursor;
OS   Apis mellifera carnica (Carniolan honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=88217;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND MASS SPECTROMETRY.
RC   TISSUE=Head, and Royal jelly;
RX   PubMed=15607651; DOI=10.1016/j.ibmb.2004.09.007;
RA   Klaudiny J., Albert S., Bachanova K., Kopernicky J., Simuth J.;
RT   "Two structurally different defensin genes, one of them encoding a novel
RT   defensin isoform, are expressed in honeybee Apis mellifera.";
RL   Insect Biochem. Mol. Biol. 35:11-22(2005).
CC   -!- FUNCTION: Found in royal jelly and in hemolymph, potent antibacterial
CC       protein against Gram-positive bacteria at low concentration.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MASS SPECTROMETRY: Mass=5515.5; Mass_error=3; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15607651};
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 1
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR   EMBL; AY333923; AAR01214.1; -; mRNA.
DR   AlphaFoldDB; Q5J8R1; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR017982; Defensin_insect.
DR   InterPro; IPR001542; Defensin_invertebrate/fungal.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   Pfam; PF01097; Defensin_2; 1.
DR   PRINTS; PR00271; DEFENSIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Cleavage on pair of basic residues;
KW   Defensin; Disulfide bond; Immunity; Innate immunity; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..43
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000394507"
FT   CHAIN           44..94
FT                   /note="Defensin-1"
FT                   /id="PRO_5000091845"
FT   MOD_RES         94
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000250"
FT   DISULFID        46..74
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT   DISULFID        60..79
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT   DISULFID        64..81
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
SQ   SEQUENCE   95 AA;  10717 MW;  8689FDC5FC9C6F5A CRC64;
     MKIYFIVGLL FMAMVAIMAA PVEDEFEPLE HFENEERADR HRRVTCDLLS FKGQVNDSAC
     AANCLSLGKA GGHCEKGVCI CRKTSFKDLW DKRFG