ID   DEFI_CALVI              Reviewed;          40 AA.
AC   C0HJX7;
DT   17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT   17-FEB-2016, sequence version 1.
DT   03-AUG-2022, entry version 10.
DE   RecName: Full=Defensin {ECO:0000303|Ref.1};
OS   Calliphora vicina (Blue blowfly) (Calliphora erythrocephala).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC   Calliphoridae; Calliphorinae; Calliphora.
OX   NCBI_TaxID=7373 {ECO:0000303|Ref.1};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, INDUCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Hemolymph {ECO:0000303|Ref.1};
RA   Chernysh S., Gordja N.A., Simnonenko N.P.;
RT   "Dia pause and immune response: induction of antimicrobial peptides
RT   synthesis in the blowfly, Calliphora vicina R.-D. (Diptera:
RT   Calliphoridae).";
RL   Entomol. Sci. 3:139-144(2000).
RN   [2] {ECO:0000305}
RP   FUNCTION, INDUCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Hemolymph {ECO:0000303|PubMed:26177023};
RX   PubMed=26177023; DOI=10.1371/journal.pone.0130788;
RA   Chernysh S., Gordya N., Suborova T.;
RT   "Insect antimicrobial peptide complexes prevent resistance development in
RT   bacteria.";
RL   PLoS ONE 10:E0130788-E0130788(2015).
CC   -!- FUNCTION: Antimicrobial peptide mostly active against Gram-positive
CC       bacteria (Ref.1, PubMed:26177023). Active against Gram-positive
CC       bacterium M.luteus and also against fungus N.crassa (Ref.1). Has no
CC       activity against Gram-negative bacterium E.coli (Ref.1). Active against
CC       M.luteus and, weakly, against E.coli (PubMed:26177023).
CC       {ECO:0000269|PubMed:26177023, ECO:0000269|Ref.1}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|Ref.1}.
CC   -!- INDUCTION: By bacterial infection. {ECO:0000269|PubMed:26177023,
CC       ECO:0000269|Ref.1}.
CC   -!- MASS SPECTROMETRY: Mass=4032; Method=MALDI;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- MASS SPECTROMETRY: Mass=4032.6; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:26177023};
CC   -!- MISCELLANEOUS: At least 3 isoforms with masses ranging from 4032 kDa to
CC       4114 kDa seem to exist. {ECO:0000269|PubMed:26177023,
CC       ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 1
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; C0HJX7; -.
DR   SMR; C0HJX7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IEA:UniProt.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR017982; Defensin_insect.
DR   InterPro; IPR001542; Defensin_invertebrate/fungal.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   Pfam; PF01097; Defensin_2; 1.
DR   PRINTS; PR00271; DEFENSIN.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Immunity; Innate immunity; Secreted.
FT   CHAIN           1..40
FT                   /note="Defensin"
FT                   /id="PRO_0000435613"
FT   DISULFID        3..30
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT   DISULFID        16..36
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT   DISULFID        20..38
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
SQ   SEQUENCE   40 AA;  4039 MW;  2A7BFED58ADFC362 CRC64;
     ATCDLLSGTG ANHSACAAHC LLRGNRGGYC NGKAVCVCRN