DEFI_DROME
ID DEFI_DROME Reviewed; 92 AA.
AC P36192; Q4V3H2; Q867E6; Q867G6; Q86BV6; Q86BV7; Q9V5F5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Defensin;
DE Flags: Precursor;
GN Name=Def; ORFNames=CG1385;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=Oregon-R;
RX PubMed=8168509; DOI=10.1111/j.1432-1033.1994.tb18730.x;
RA Dimarcq J.-L., Hoffmann D., Meister M., Bulet P., Lanot R.,
RA Reichhart J.-M., Hoffmann J.A.;
RT "Characterization and transcriptional profiles of a Drosophila gene
RT encoding an insect defensin. A study in insect immunity.";
RL Eur. J. Biochem. 221:201-209(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=2CPA1, 2CPA103, 2CPA105, 2CPA118, 2CPA12, 2CPA122, 2CPA129, 2CPA14,
RC 2CPA43, 2CPA46, 2CPA51, and 2CPA7;
RX PubMed=12716986; DOI=10.1093/molbev/msg109;
RA Lazzaro B.P., Clark A.G.;
RT "Molecular population genetics of inducible antibacterial peptide genes in
RT Drosophila melanogaster.";
RL Mol. Biol. Evol. 20:914-923(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Pak S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY BACTERIA, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Hemolymph {ECO:0000303|PubMed:16510152};
RX PubMed=16510152; DOI=10.1016/j.jinsphys.2005.12.007;
RA Verleyen P., Baggerman G., D'Hertog W., Vierstraete E., Husson S.J.,
RA Schoofs L.;
RT "Identification of new immune induced molecules in the haemolymph of
RT Drosophila melanogaster by 2D-nanoLC MS/MS.";
RL J. Insect Physiol. 52:379-388(2006).
CC -!- FUNCTION: Responsible for the anti Gram-positive activity of immune
CC hemolymph. Expressed in the absence of immune challenge during
CC metamorphosis. {ECO:0000269|PubMed:8168509}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00710,
CC ECO:0000269|PubMed:16510152, ECO:0000269|PubMed:8168509}.
CC -!- TISSUE SPECIFICITY: Hemolymph (at protein level).
CC {ECO:0000269|PubMed:16510152}.
CC -!- INDUCTION: By bacterial infection (at protein level).
CC {ECO:0000269|PubMed:16510152}.
CC -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 1
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z27247; CAA81760.1; -; mRNA.
DR EMBL; AY224631; AAO72490.1; -; Genomic_DNA.
DR EMBL; AY224632; AAO72491.1; -; Genomic_DNA.
DR EMBL; AY224633; AAO72492.1; -; Genomic_DNA.
DR EMBL; AY224634; AAO72493.1; -; Genomic_DNA.
DR EMBL; AY224635; AAO72494.1; -; Genomic_DNA.
DR EMBL; AY224636; AAO72495.1; -; Genomic_DNA.
DR EMBL; AY224637; AAO72496.1; -; Genomic_DNA.
DR EMBL; AY224638; AAO72497.1; -; Genomic_DNA.
DR EMBL; AY224639; AAO72498.1; -; Genomic_DNA.
DR EMBL; AY224640; AAO72499.1; -; Genomic_DNA.
DR EMBL; AY224641; AAO72500.1; -; Genomic_DNA.
DR EMBL; AY224642; AAO72501.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF58855.1; -; Genomic_DNA.
DR EMBL; BT023384; AAY55800.1; -; mRNA.
DR PIR; S43228; S43228.
DR RefSeq; NP_523672.1; NM_078948.3.
DR AlphaFoldDB; P36192; -.
DR BioGRID; 61875; 2.
DR IntAct; P36192; 6.
DR STRING; 7227.FBpp0087518; -.
DR TCDB; 1.C.47.1.1; the insect/fungal defensin (insect/fungal defensin) family.
DR PaxDb; P36192; -.
DR DNASU; 36047; -.
DR EnsemblMetazoa; FBtr0088432; FBpp0087518; FBgn0010385.
DR GeneID; 36047; -.
DR KEGG; dme:Dmel_CG1385; -.
DR UCSC; CG1385-RA; d. melanogaster.
DR CTD; 36047; -.
DR FlyBase; FBgn0010385; Def.
DR VEuPathDB; VectorBase:FBgn0010385; -.
DR eggNOG; ENOG502SD3P; Eukaryota.
DR HOGENOM; CLU_174272_0_0_1; -.
DR InParanoid; P36192; -.
DR OMA; HSACAVH; -.
DR OrthoDB; 1600872at2759; -.
DR PhylomeDB; P36192; -.
DR BioGRID-ORCS; 36047; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36047; -.
DR PRO; PR:P36192; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0010385; Expressed in oviduct (Drosophila) and 17 other tissues.
DR ExpressionAtlas; P36192; baseline and differential.
DR Genevisible; P36192; DM.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0019731; P:antibacterial humoral response; IEP:FlyBase.
DR GO; GO:0042742; P:defense response to bacterium; IBA:GO_Central.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:FlyBase.
DR GO; GO:0006959; P:humoral immune response; IEP:FlyBase.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0009617; P:response to bacterium; IDA:FlyBase.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR001542; Defensin_invertebrate/fungal.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR Pfam; PF01097; Defensin_2; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Cleavage on pair of basic residues; Defensin;
KW Disulfide bond; Immunity; Innate immunity; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT PROPEP 21..52
FT /id="PRO_0000006742"
FT CHAIN 53..92
FT /note="Defensin"
FT /id="PRO_0000006743"
FT DISULFID 55..82
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT DISULFID 68..88
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT DISULFID 72..90
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710"
FT VARIANT 6
FT /note="L -> P (in strain: 2CPA7 and 2CPA14)"
FT VARIANT 15
FT /note="A -> T (in strain: 2CPA12, 2CPA43, 2CPA46, 2CPA51,
FT 2CPA103 and 2CPA105)"
FT VARIANT 17
FT /note="V -> M (in strain: 2CPA1, 2CPA12, 2CPA43, 2CPA46,
FT 2CPA51, 2CPA103 and 2CPA105)"
FT VARIANT 38
FT /note="E -> D (in strain: 2CPA7 and 2CPA14)"
SQ SEQUENCE 92 AA; 10190 MW; 3E092BBAC640EDF6 CRC64;
MKFFVLVAIA FALLACVAQA QPVSDVDPIP EDHVLVHEDA HQEVLQHSRQ KRATCDLLSK
WNWNHTACAG HCIAKGFKGG YCNDKAVCVC RN
