ID   DEFLO_HAELO             Reviewed;          78 AA.
AC   B2MW54;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Longicornsin {ECO:0000303|PubMed:20027626, ECO:0000312|EMBL:ACC95997.1};
DE   Flags: Precursor;
GN   Name=sGDLP {ECO:0000312|EMBL:ACC95997.1};
OS   Haemaphysalis longicornis (Bush tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Haemaphysalinae;
OC   Haemaphysalis.
OX   NCBI_TaxID=44386;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:ACC95997.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 32-78, FUNCTION, TISSUE
RP   SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Salivary gland {ECO:0000312|EMBL:ACC95997.1};
RX   PubMed=20027626; DOI=10.1002/pro.317;
RA   Lu X., Che Q., Lv Y., Wang M., Lu Z., Feng F., Liu J., Yu H.;
RT   "A novel defensin-like peptide from salivary glands of the hard tick,
RT   Haemaphysalis longicornis.";
RL   Protein Sci. 19:392-397(2010).
CC   -!- FUNCTION: Has antibacterial activity against the Gram-positive bacteria
CC       S.aureus ATCC2592 (MIC=0.8 ug/ml), S.aureus 6A (MIC=0.8 ug/ml) and
CC       S.aureus 15A (MIC=1.6 ug/ml), and against the Gram-negative bacteria
CC       E.coli ATCC 25922 (MIC=3.2 ug/ml), E.coli 23A (MIC=6.4 ug/ml), E.coli
CC       27A (MIC=6.4 ug/ml), P.aeruginosa 3A (MIC=3.2 ug/ml), P.aeruginosa 7A
CC       (MIC=0.8 ug/ml) and H.pylori NCTC11637 (MIC=6.4 ug/ml). Has antifungal
CC       activity against C.albidus ATCC2002 (MIC=25.6 ug/ml). Very low
CC       hemolytic activity against rabbit erythrocytes.
CC       {ECO:0000269|PubMed:20027626}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P80571}.
CC   -!- TISSUE SPECIFICITY: Salivary glands (at protein level).
CC       {ECO:0000269|PubMed:20027626}.
CC   -!- MASS SPECTROMETRY: Mass=5370.1; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:20027626};
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 2
CC       subfamily. {ECO:0000255}.
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DR   EMBL; EU627689; ACC95997.1; -; mRNA.
DR   AlphaFoldDB; B2MW54; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0044179; P:hemolysis in another organism; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Cleavage on pair of basic residues; Cytolysis;
KW   Defensin; Direct protein sequencing; Disulfide bond; Fungicide; Hemolysis;
KW   Immunity; Innate immunity; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..29
FT                   /evidence="ECO:0000255, ECO:0000269|PubMed:20027626"
FT                   /id="PRO_0000403320"
FT   CHAIN           32..78
FT                   /note="Longicornsin"
FT                   /evidence="ECO:0000269|PubMed:20027626"
FT                   /id="PRO_5000365774"
FT   DISULFID        35..58
FT                   /evidence="ECO:0000250|UniProtKB:P80571"
FT   DISULFID        43..63
FT                   /evidence="ECO:0000250|UniProtKB:P80571"
FT   DISULFID        47..65
FT                   /evidence="ECO:0000250|UniProtKB:P80571"
SQ   SEQUENCE   78 AA;  8837 MW;  0F531A011DA9B859 CRC64;
     MAESTTTCFL LLVTGYVTAV MSEEAHLRSR RDFGCGQGMI FMCQRRCMRL YPGSTGFCRG
     FRCMCDTHIP LRPPFMVG