DEFN_ARCDE
ID DEFN_ARCDE Reviewed; 44 AA.
AC P84156;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 25-MAY-2022, entry version 58.
DE RecName: Full=Defensin ARD1;
OS Archaeoprepona demophon (One-spotted leafwing butterfly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Papilionoidea;
OC Nymphalidae; Charaxinae; Preponini; Archaeoprepona.
OX NCBI_TaxID=191427;
RN [1] {ECO:0000305, ECO:0000312|PDB:1OZZ}
RP PROTEIN SEQUENCE, STRUCTURE BY NMR, FUNCTION, MASS SPECTROMETRY, AND
RP MUTAGENESIS OF ASN-19 AND ALA-36.
RC TISSUE=Hemolymph {ECO:0000269|PubMed:14978308};
RX PubMed=14978308; DOI=10.1110/ps.03404404;
RA Landon C., Barbault F., Legrain M., Menin L., Guenneugues M., Schott V.,
RA Vovelle F., Dimarcq J.-L.;
RT "Lead optimization of antifungal peptides with 3D NMR structures
RT analysis.";
RL Protein Sci. 13:703-713(2004).
CC -!- FUNCTION: Possesses potent anti-fungal activity.
CC {ECO:0000269|PubMed:14978308}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000255|PROSITE-ProRule:PRU00710,
CC ECO:0000269|PubMed:14978308}.
CC -!- MASS SPECTROMETRY: Mass=4803.4; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:14978308};
CC -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 2
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR PDB; 1OZZ; NMR; -; A=1-44.
DR PDB; 1P00; NMR; -; A=1-44.
DR PDB; 1P0A; NMR; -; A=1-44.
DR PDBsum; 1OZZ; -.
DR PDBsum; 1P00; -.
DR PDBsum; 1P0A; -.
DR AlphaFoldDB; P84156; -.
DR SMR; P84156; -.
DR EvolutionaryTrace; P84156; -.
DR GO; GO:0005576; C:extracellular region; TAS:UniProtKB.
DR GO; GO:0019732; P:antifungal humoral response; IDA:UniProtKB.
DR GO; GO:0006952; P:defense response; TAS:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR001542; Defensin_invertebrate/fungal.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antimicrobial; Defensin; Direct protein sequencing;
KW Disulfide bond; Fungicide; Immunity; Innate immunity; Secreted.
FT CHAIN 1..44
FT /note="Defensin ARD1"
FT /id="PRO_0000074476"
FT DISULFID 7..32
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT ECO:0000269|PubMed:14978308"
FT DISULFID 18..40
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT ECO:0000269|PubMed:14978308"
FT DISULFID 22..42
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00710,
FT ECO:0000269|PubMed:14978308"
FT MUTAGEN 19
FT /note="N->R: Improves antifungal activity."
FT /evidence="ECO:0000269|PubMed:14978308"
FT MUTAGEN 36
FT /note="A->L: Improves antifungal activity."
FT /evidence="ECO:0000269|PubMed:14978308"
FT STRAND 2..8
FT /evidence="ECO:0007829|PDB:1OZZ"
FT STRAND 11..14
FT /evidence="ECO:0007829|PDB:1OZZ"
FT HELIX 18..25
FT /evidence="ECO:0007829|PDB:1OZZ"
FT STRAND 27..34
FT /evidence="ECO:0007829|PDB:1OZZ"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:1OZZ"
FT STRAND 39..43
FT /evidence="ECO:0007829|PDB:1OZZ"
SQ SEQUENCE 44 AA; 4803 MW; 367DC7B793DCFCD5 CRC64;
DKLIGSCVWG AVNYTSNCNA ECKRRGYKGG HCGSFANVNC WCET
