DEFN_HELVI
ID DEFN_HELVI Reviewed; 44 AA.
AC P81544;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Defensin heliomicin;
OS Heliothis virescens (Tobacco budworm moth).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Noctuoidea;
OC Noctuidae; Heliothinae; Heliothis.
OX NCBI_TaxID=7102;
RN [1]
RP PROTEIN SEQUENCE, CHARACTERIZATION, AND MASS SPECTROMETRY.
RC TISSUE=Hemolymph;
RX PubMed=10092609; DOI=10.1074/jbc.274.14.9320;
RA Lamberty M., Ades S., Uttenweiler-Joseph S., Brookhart G., Bushey D.,
RA Hoffmann J.A., Bulet P.;
RT "Insect immunity. Isolation from the lepidopteran Heliothis virescens of a
RT novel insect defensin with potent antifungal activity.";
RL J. Biol. Chem. 274:9320-9326(1999).
RN [2]
RP STRUCTURE BY NMR.
RX PubMed=11580275; DOI=10.1021/bi0103563;
RA Lamberty M., Caille A., Landon C., Tassin-Moindrot S., Hetru C., Bulet P.,
RA Vovelle F.;
RT "Solution structures of the antifungal heliomicin and a selected variant
RT with both antibacterial and antifungal activities.";
RL Biochemistry 40:11995-12003(2001).
CC -!- FUNCTION: This peptide has potent anti-fungal activity. Has no activity
CC against Gram-negative and Gram-positive bacteria.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- MASS SPECTROMETRY: Mass=4784.8; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10092609};
CC -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 2
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR PDB; 1I2U; NMR; -; A=1-44.
DR PDB; 1I2V; NMR; -; A=1-44.
DR PDBsum; 1I2U; -.
DR PDBsum; 1I2V; -.
DR AlphaFoldDB; P81544; -.
DR SMR; P81544; -.
DR EvolutionaryTrace; P81544; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR001542; Defensin_invertebrate/fungal.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antimicrobial; Defensin; Direct protein sequencing;
KW Disulfide bond; Fungicide; Immunity; Innate immunity; Secreted.
FT CHAIN 1..44
FT /note="Defensin heliomicin"
FT /id="PRO_0000074477"
FT DISULFID 7..32
FT DISULFID 18..40
FT DISULFID 22..42
FT STRAND 2..6
FT /evidence="ECO:0007829|PDB:1I2U"
FT STRAND 8..11
FT /evidence="ECO:0007829|PDB:1I2U"
FT HELIX 18..24
FT /evidence="ECO:0007829|PDB:1I2U"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:1I2U"
FT STRAND 34..37
FT /evidence="ECO:0007829|PDB:1I2U"
FT STRAND 39..42
FT /evidence="ECO:0007829|PDB:1I2U"
SQ SEQUENCE 44 AA; 4790 MW; 301DA1BD3376FCD5 CRC64;
DKLIGSCVWG AVNYTSDCNG ECKRRGYKGG HCGSFANVNC WCET
