3BHS_VACCW
ID 3BHS_VACCW Reviewed; 346 AA.
AC P26670; Q76ZN3;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase;
DE Short=3-beta-HSD;
DE Includes:
DE RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE EC=1.1.1.145;
DE AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE AltName: Full=Progesterone reductase;
DE Includes:
DE RecName: Full=Steroid Delta-isomerase;
DE EC=5.3.3.1;
DE AltName: Full=Delta-5-3-ketosteroid isomerase;
GN OrderedLocusNames=VACWR170; ORFNames=SALF7L;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1870190; DOI=10.1128/jvi.65.9.4598-4608.1991;
RA Blasco R., Cole N.B., Moss B.;
RT "Sequence analysis, expression, and deletion of a vaccinia virus gene
RT encoding a homolog of profilin, a eukaryotic actin-binding protein.";
RL J. Virol. 65:4598-4608(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2045793; DOI=10.1099/0022-1317-72-6-1349;
RA Smith G.L., Chan Y.S., Howard S.T.;
RT "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the
RT right inverted terminal repeat.";
RL J. Gen. Virol. 72:1349-1376(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=9722876; DOI=10.1007/s007050050377;
RA Sroller V., Kutinova L., Nemeckova S., Simonova V., Vonka V.;
RT "Effect of 3-beta-hydroxysteroid dehydrogenase gene deletion on virulence
RT and immunogenicity of different vaccinia viruses and their recombinants.";
RL Arch. Virol. 143:1311-1320(1998).
RN [5]
RP FUNCTION.
RX PubMed=1582424; DOI=10.1002/j.1460-2075.1992.tb05251.x;
RA Moore J.B., Smith G.L.;
RT "Steroid hormone synthesis by a vaccinia enzyme: a new type of virus
RT virulence factor.";
RL EMBO J. 11:1973-1980(1992).
CC -!- FUNCTION: Catalyzes the oxidative conversion of Delta(5)-ene-3-beta-
CC hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-
CC beta-HSD enzymatic system plays a crucial role in the biosynthesis of
CC all classes of hormonal steroids. During viral infection, steroid
CC production contributes to virulence by inhibiting the host inflammatory
CC response. {ECO:0000269|PubMed:1582424, ECO:0000269|PubMed:9722876}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-
CC steroid + H(+) + NADH; Xref=Rhea:RHEA:24076, ChEBI:CHEBI:1722,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47907, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.1.1.145;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid;
CC Xref=Rhea:RHEA:14709, ChEBI:CHEBI:47907, ChEBI:CHEBI:47909;
CC EC=5.3.3.1;
CC -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC -!- SIMILARITY: Belongs to the 3-beta-HSD family. {ECO:0000305}.
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DR EMBL; M72474; AAA48311.1; -; Genomic_DNA.
DR EMBL; D11079; BAA01818.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89449.1; -; Genomic_DNA.
DR PIR; E40897; WMVZ2W.
DR RefSeq; YP_233052.1; NC_006998.1.
DR SMR; P26670; -.
DR DNASU; 3707700; -.
DR GeneID; 3707700; -.
DR KEGG; vg:3707700; -.
DR UniPathway; UPA00062; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0102294; F:cholesterol dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0004769; F:steroid delta-isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01073; 3Beta_HSD; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 3: Inferred from homology;
KW Host-virus interaction; Isomerase; Multifunctional enzyme; NAD;
KW Oxidoreductase; Reference proteome; Steroidogenesis; Viral immunoevasion.
FT CHAIN 1..346
FT /note="3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-
FT isomerase"
FT /id="PRO_0000087795"
FT ACT_SITE 147
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 151
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 346 AA; 39396 MW; 3107A2B7F442CCD9 CRC64;
MAVYAVTGGA GFLGRYIVKL LISADDVQEI RVIDIVEDPQ PITSKVKVIN YIQCDINDFD
KVREALDGVN LIIHTAALVD VFGKYTDNEI MKVNYYGTQT ILAACVDLGI KYLIYTSSME
AIGPNKHGDP FIGHEHTLYD ISPGHVYAKS KRMAEQLVMK ANNSVIMNGA KLYTCCLRPT
GIYGEGDKLT KVFYEQCKQH GNIMYRTVDD DAVHSRVYVG NVAWMHVLAA KYIQYPGSEI
KGNAYFCYDY SPSCSYDMFN LLLMKPLGIE QGSRIPRWML KMYACKNDMK RILFRKPSLL
NNYTLKISNT TFEVRTNNAE LDFNYSPIFN VDVAFERTRK WLEESE
