ID   DEF_GYMCO               Reviewed;          75 AA.
AC   B5LZ79;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   14-OCT-2008, sequence version 1.
DT   25-MAY-2022, entry version 21.
DE   RecName: Full=Defensin-like protein {ECO:0000250|UniProtKB:Q09198};
DE   AltName: Full=Gamma-thionin {ECO:0000312|EMBL:ACH67608.1};
DE   Flags: Precursor;
OS   Gymnadenia conopsea (Fragrant orchid) (Orchis conopsea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Orchidoideae; Orchideae; Orchidinae; Gymnadenia.
OX   NCBI_TaxID=59324;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:ACH67608.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Li X.-F., Zhou J.-P., Liu Y., Yuan J.-B., Hao J.-L., Feng J., Ren Z.-L.;
RT   "Prokaryotic expression, purification and western blotting of gamma-thionin
RT   of Gymnadenia conopsea R.Br.";
RL   Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305, ECO:0000312|EMBL:ACH67608.1}
RP   PROTEIN SEQUENCE OF 31-75.
RC   TISSUE=Shoot {ECO:0000269|Ref.1};
RA   Li X.-F., Zhou J.-P., Liu Y., Yuan J.-B., Hao J.-L., Feng J., Ren Z.-L.;
RT   "Prokaryotic expression, purification and western blotting of gamma-thionin
RT   of Gymnadenia conopsea R.Br.";
RL   Submitted (JUL-2008) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:O65740}.
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000255}.
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DR   EMBL; EU925640; ACH67608.1; -; mRNA.
DR   AlphaFoldDB; B5LZ79; -.
DR   SMR; B5LZ79; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW   Plant defense; Secreted; Signal.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000269|Ref.1, ECO:0000269|Ref.2"
FT   CHAIN           31..75
FT                   /note="Defensin-like protein"
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000388751"
FT   DISULFID        33..75
FT                   /evidence="ECO:0000250|UniProtKB:Q09198"
FT   DISULFID        42..62
FT                   /evidence="ECO:0000250|UniProtKB:Q09198"
FT   DISULFID        48..69
FT                   /evidence="ECO:0000250|UniProtKB:Q09198"
FT   DISULFID        52..71
FT                   /evidence="ECO:0000250|UniProtKB:Q09198"
SQ   SEQUENCE   75 AA;  8530 MW;  4289A9A6B2B575B0 CRC64;
     MVLNNKTALW FMTLVLLLLS SREMVPGAEA RLCRVRGTRG HCFNDHGCDK VCTREGFVRG
     KCNGILRRCI CDRQC