3BHS_VAR67
ID 3BHS_VAR67 Reviewed; 210 AA.
AC Q89187;
DT 21-SEP-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase;
DE Short=3-beta-HSD;
DE Includes:
DE RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE EC=1.1.1.145;
DE AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE AltName: Full=Progesterone reductase;
DE Includes:
DE RecName: Full=Steroid Delta-isomerase;
DE EC=5.3.3.1;
DE AltName: Full=Delta-5-3-ketosteroid isomerase;
GN Name=A49L;
OS Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX NCBI_TaxID=587200;
OH NCBI_TaxID=9606; Homo sapiens (Human).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT "Genes of variola and vaccinia viruses necessary to overcome the host
RT protective mechanisms.";
RL FEBS Lett. 319:80-83(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7856312; DOI=10.1016/0168-1702(94)90125-2;
RA Shchelkunov S.N., Blinov V.M., Resenchuk S.M., Totmenin A.V., Olenina L.V.,
RA Chirikova G.B., Sandakhchiev L.S.;
RT "Analysis of the nucleotide sequence of 53 kbp from the right terminus of
RT the genome of variola major virus strain India-1967.";
RL Virus Res. 34:207-236(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7597802; DOI=10.1007/bf01702879;
RA Shchelkunov S.N., Totmenin A.V.;
RT "Two types of deletions in orthopoxvirus genomes.";
RL Virus Genes 9:231-245(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8725113; DOI=10.1016/0168-1702(95)01269-9;
RA Shchelkunov S.N., Totmenin A.V., Sandakhchiev L.S.;
RT "Analysis of the nucleotide sequence of 23.8 kbp from the left terminus of
RT the genome of variola major virus strain India-1967.";
RL Virus Res. 40:169-183(1996).
CC -!- FUNCTION: Catalyzes the oxidative conversion of Delta(5)-ene-3-beta-
CC hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-
CC beta-HSD enzymatic system plays a crucial role in the biosynthesis of
CC all classes of hormonal steroids. During viral infection, steroid
CC production contributes to virulence by inhibiting the host inflammatory
CC response (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-
CC steroid + H(+) + NADH; Xref=Rhea:RHEA:24076, ChEBI:CHEBI:1722,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47907, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.1.1.145;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid;
CC Xref=Rhea:RHEA:14709, ChEBI:CHEBI:47907, ChEBI:CHEBI:47909;
CC EC=5.3.3.1;
CC -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC -!- SIMILARITY: Belongs to the 3-beta-HSD family. {ECO:0000305}.
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DR EMBL; X69198; CAA49096.1; -; Genomic_DNA.
DR PIR; E36853; E36853.
DR PIR; JQ1850; JQ1850.
DR RefSeq; NP_042199.1; NC_001611.1.
DR PRIDE; Q89187; -.
DR GeneID; 1486530; -.
DR KEGG; vg:1486530; -.
DR UniPathway; UPA00062; -.
DR Proteomes; UP000002060; Genome.
DR GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0102294; F:cholesterol dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0004769; F:steroid delta-isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01073; 3Beta_HSD; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 3: Inferred from homology;
KW Host-virus interaction; Isomerase; Multifunctional enzyme; NAD;
KW Oxidoreductase; Reference proteome; Steroidogenesis; Viral immunoevasion.
FT CHAIN 1..210
FT /note="3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-
FT isomerase"
FT /id="PRO_0000412627"
FT ACT_SITE 29
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 33
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 210 AA; 24163 MW; AAAEEB99C00AF08A CRC64;
MEAIGPNKHG NPFIGHEHTL YDISPGHVYA KSKRMVEQLV TKANNSVIMN GAKLYTCCLR
PTGIYGGGDK LTKVFYEQCT QHGNIMYRTV DDDAVHSRVY VGNVAWMHVL AVKYIQYPGS
EIKGNAYFCY GYSPSCSYDM FNLLLMKPLG IEQGSRIPRW MLKMYTCKND MKRILFRKPS
LLNNYTLKIS NTTFEARQQC RTRFQLLPYL