ID   DEF_TOBAC               Reviewed;         105 AA.
AC   P32026;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Defensin-like protein;
DE   AltName: Full=Flower-specific gamma-thionin;
DE   Flags: Precursor;
GN   Name=FST;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Wisconsin 38; TISSUE=Flower bud;
RX   PubMed=1495489; DOI=10.1007/bf00272349;
RA   Gu Q., Kawata E.E., Morse M.-J., Wu H.-M., Cheung A.Y.;
RT   "A flower-specific cDNA encoding a novel thionin in tobacco.";
RL   Mol. Gen. Genet. 234:89-96(1992).
RN   [2]
RP   STRUCTURE BY NMR OF 26-72, AND DISULFIDE BONDS.
RX   PubMed=12473460; DOI=10.1016/s0022-2836(02)01103-8;
RA   Lay F.T., Schirra H.J., Scanlon M.J., Anderson M.A., Craik D.J.;
RT   "The three-dimensional solution structure of NaD1, a new floral defensin
RT   from Nicotiana alata and its application to a homology model of the crop
RT   defense protein alfAFP.";
RL   J. Mol. Biol. 325:175-188(2003).
CC   -!- FUNCTION: Involved in floral organogenesis. May play a protective role
CC       in flowers by protecting the reproductive organs from potential
CC       pathogen attack.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. Vacuole
CC       {ECO:0000250}. Note=Possibly the cell wall or vacuole.
CC   -!- TISSUE SPECIFICITY: Flower. Found in petals, stamen and pistils, but
CC       not in sepals. In particular, accumulation in a configuration
CC       surrounding the inner reproductive whorls.
CC   -!- DEVELOPMENTAL STAGE: Accumulates in developing flowers and its level
CC       drops as flowers mature.
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC   -!- CAUTION: Was initially thought to be a thionin.
CC       {ECO:0000305|PubMed:1495489}.
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DR   EMBL; Z11748; CAA77806.1; -; mRNA.
DR   PIR; S23574; S23574.
DR   RefSeq; XP_016515032.1; XM_016659546.1.
DR   PDB; 1MR4; NMR; -; A=26-72.
DR   PDBsum; 1MR4; -.
DR   AlphaFoldDB; P32026; -.
DR   SMR; P32026; -.
DR   TCDB; 1.C.45.1.2; the plant defensin (plant defensin) family.
DR   GeneID; 107831752; -.
DR   KEGG; nta:107831752; -.
DR   OMA; CISEKFT; -.
DR   OrthoDB; 1544394at2759; -.
DR   EvolutionaryTrace; P32026; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   CDD; cd00107; Knot1; 1.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   PRINTS; PR00288; PUROTHIONIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antimicrobial; Cell wall; Disulfide bond; Fungicide;
KW   Plant defense; Reference proteome; Secreted; Signal; Vacuole.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..105
FT                   /note="Defensin-like protein"
FT                   /id="PRO_0000007057"
FT   DISULFID        28..72
FT                   /evidence="ECO:0000269|PubMed:12473460"
FT   DISULFID        39..59
FT                   /evidence="ECO:0000269|PubMed:12473460"
FT   DISULFID        45..66
FT                   /evidence="ECO:0000269|PubMed:12473460"
FT   DISULFID        49..68
FT                   /evidence="ECO:0000269|PubMed:12473460"
FT   STRAND          28..31
FT                   /evidence="ECO:0007829|PDB:1MR4"
FT   HELIX           42..51
FT                   /evidence="ECO:0007829|PDB:1MR4"
FT   STRAND          55..59
FT                   /evidence="ECO:0007829|PDB:1MR4"
FT   STRAND          63..70
FT                   /evidence="ECO:0007829|PDB:1MR4"
SQ   SEQUENCE   105 AA;  11750 MW;  DA7F4511F0651BC3 CRC64;
     MARSLCFMAF AILAMMLFVA YEVQARECKT ESNTFPGICI TKPPCRKACI SEKFTDGHCS
     KLLRRCLCTK PCVFDEKMIK TGAETLVEEA KTLAAALLEE EIMDN