ID   DEGM_BACB2              Reviewed;         373 AA.
AC   Q03069;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Sensor protein DegM;
DE            EC=2.7.13.3;
GN   Name=degM;
OS   Bacillus sp. (strain B21-2).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=69001;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Masui A., Fujiwara N., Takagi M., Imanaka T.;
RT   "Cloning and nucleotide sequence of degM, the regulatory gene, degM, for
RT   minor protease in Bacillus subtilis.";
RL   J. Ferment. Bioeng. 74:230-233(1992).
CC   -!- FUNCTION: Involved in a sensory transduction pathway that enhances the
CC       production of minor proteases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC         histidine.; EC=2.7.13.3;
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
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DR   EMBL; D10690; BAA01532.1; -; Genomic_DNA.
DR   PIR; I39833; I39833.
DR   AlphaFoldDB; Q03069; -.
DR   SMR; Q03069; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000155; F:phosphorelay sensor kinase activity; IEA:InterPro.
DR   CDD; cd00082; HisKA; 1.
DR   Gene3D; 3.30.565.10; -; 1.
DR   InterPro; IPR003594; HATPase_C.
DR   InterPro; IPR036890; HATPase_C_sf.
DR   InterPro; IPR005467; His_kinase_dom.
DR   InterPro; IPR003661; HisK_dim/P.
DR   InterPro; IPR036097; HisK_dim/P_sf.
DR   InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR   Pfam; PF02518; HATPase_c; 1.
DR   Pfam; PF00512; HisKA; 1.
DR   PRINTS; PR00344; BCTRLSENSOR.
DR   SMART; SM00387; HATPase_c; 1.
DR   SMART; SM00388; HisKA; 1.
DR   SUPFAM; SSF47384; SSF47384; 1.
DR   SUPFAM; SSF55874; SSF55874; 1.
DR   PROSITE; PS50109; HIS_KIN; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW   Phosphoprotein; Transferase; Transmembrane; Transmembrane helix;
KW   Two-component regulatory system.
FT   CHAIN           1..373
FT                   /note="Sensor protein DegM"
FT                   /id="PRO_0000074754"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        122..142
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          170..370
FT                   /note="Histidine kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT   MOD_RES         173
FT                   /note="Phosphohistidine; by autocatalysis"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ   SEQUENCE   373 AA;  41614 MW;  6494279E0879294B CRC64;
     MSGAVLLCLF FSINVGNIQW DLRYIPILLA FLYGGKRAGW GVAAIAVVGR IGQGGDLFLL
     GVVLIVLTAL FYALCVNRFY MLPPRWSRIR YASLLVILPA TIQTFGTLYL INYENHLSES
     WVAGWLYIVF LVVTVVLVTY LFETLLEKER IVAELVATEK DFTKGELAAS IAHEVRNPLT
     VVKGFVQLLS EDKQHAEYHK LILSELDRAE SIIYEFLNTT RPQTNATFHL NETAREVVAL
     LTPYAQERSI QLTIGTCEQA IVNGNENKVK QALMNFVKNG IEASNEGDTV TIQLDRLKDR
     AQIEINDNGV GMSRQQLKQL GTAYFTTKES GNGIGTMVSI RIVEMMNGMV TFKSKPGKGT
     KVVLSLPIEK ENE