DEGP8_ARATH
ID DEGP8_ARATH Reviewed; 448 AA.
AC Q9LU10;
DT 18-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 145.
DE RecName: Full=Protease Do-like 8, chloroplastic;
DE EC=3.4.21.-;
DE Flags: Precursor;
GN Name=DEGP8; OrderedLocusNames=At5g39830; ORFNames=K13H13.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP PROTEIN SEQUENCE OF 91-113, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=11719511; DOI=10.1074/jbc.m108575200;
RA Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P.,
RA Kieselbach T.;
RT "Proteome map of the chloroplast lumen of Arabidopsis thaliana.";
RL J. Biol. Chem. 277:8354-8365(2002).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11299370; DOI=10.1104/pp.125.4.1912;
RA Adam Z., Adamska I., Nakabayashi K., Ostersetzer O., Haussuhl K.,
RA Manuell A., Zheng B., Vallon O., Rodermel S.R., Shinozaki K., Clarke A.K.;
RT "Chloroplast and mitochondrial proteases in Arabidopsis. A proposed
RT nomenclature.";
RL Plant Physiol. 125:1912-1918(2001).
CC -!- FUNCTION: Probable serine protease.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen
CC {ECO:0000269|PubMed:11719511}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LU10-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the peptidase S1C family. {ECO:0000305}.
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DR EMBL; AB024023; BAA98101.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94480.1; -; Genomic_DNA.
DR EMBL; AY056381; AAL08237.1; -; mRNA.
DR EMBL; AY090319; AAL90980.1; -; mRNA.
DR RefSeq; NP_568575.1; NM_123346.5. [Q9LU10-1]
DR PDB; 4IC6; X-ray; 2.00 A; A/B/C=91-448.
DR PDBsum; 4IC6; -.
DR AlphaFoldDB; Q9LU10; -.
DR SMR; Q9LU10; -.
DR STRING; 3702.AT5G39830.1; -.
DR MEROPS; S01.474; -.
DR PaxDb; Q9LU10; -.
DR PRIDE; Q9LU10; -.
DR EnsemblPlants; AT5G39830.1; AT5G39830.1; AT5G39830. [Q9LU10-1]
DR GeneID; 833979; -.
DR Gramene; AT5G39830.1; AT5G39830.1; AT5G39830. [Q9LU10-1]
DR KEGG; ath:AT5G39830; -.
DR Araport; AT5G39830; -.
DR TAIR; locus:2151916; AT5G39830.
DR eggNOG; KOG1320; Eukaryota.
DR HOGENOM; CLU_020120_2_0_1; -.
DR InParanoid; Q9LU10; -.
DR OMA; SSYPEQD; -.
DR PhylomeDB; Q9LU10; -.
DR BRENDA; 3.4.21.107; 399.
DR PRO; PR:Q9LU10; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LU10; baseline and differential.
DR Genevisible; Q9LU10; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009543; C:chloroplast thylakoid lumen; IDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0031977; C:thylakoid lumen; HDA:TAIR.
DR GO; GO:0008233; F:peptidase activity; IDA:TAIR.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0010206; P:photosystem II repair; IMP:TAIR.
DR GO; GO:0006508; P:proteolysis; IDA:TAIR.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001940; Peptidase_S1C.
DR Pfam; PF13180; PDZ_2; 1.
DR PRINTS; PR00834; PROTEASES2C.
DR SMART; SM00228; PDZ; 1.
DR SUPFAM; SSF50156; SSF50156; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Chloroplast; Direct protein sequencing;
KW Hydrolase; Plastid; Protease; Reference proteome; Serine protease;
KW Thylakoid; Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT ?..90
FT /note="Thylakoid"
FT /evidence="ECO:0000269|PubMed:11719511"
FT CHAIN 91..448
FT /note="Protease Do-like 8, chloroplastic"
FT /id="PRO_0000026942"
FT DOMAIN 336..433
FT /note="PDZ"
FT REGION 152..333
FT /note="Serine protease"
FT ACT_SITE 171
FT /note="Charge relay system"
FT /evidence="ECO:0000255"
FT ACT_SITE 214
FT /note="Charge relay system"
FT /evidence="ECO:0000255"
FT ACT_SITE 292
FT /note="Charge relay system"
FT /evidence="ECO:0000255"
FT HELIX 114..127
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 130..135
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 155..159
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 165..168
FT /evidence="ECO:0007829|PDB:4IC6"
FT HELIX 170..173
FT /evidence="ECO:0007829|PDB:4IC6"
FT HELIX 174..178
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 186..193
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 199..210
FT /evidence="ECO:0007829|PDB:4IC6"
FT TURN 211..214
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 215..219
FT /evidence="ECO:0007829|PDB:4IC6"
FT TURN 224..226
FT /evidence="ECO:0007829|PDB:4IC6"
FT HELIX 235..237
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 243..248
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 250..253
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 256..271
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 274..285
FT /evidence="ECO:0007829|PDB:4IC6"
FT TURN 289..293
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 294..298
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 303..307
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 313..315
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 321..325
FT /evidence="ECO:0007829|PDB:4IC6"
FT HELIX 326..339
FT /evidence="ECO:0007829|PDB:4IC6"
FT HELIX 354..359
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 364..370
FT /evidence="ECO:0007829|PDB:4IC6"
FT HELIX 376..380
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 398..402
FT /evidence="ECO:0007829|PDB:4IC6"
FT HELIX 410..417
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 425..432
FT /evidence="ECO:0007829|PDB:4IC6"
FT STRAND 435..442
FT /evidence="ECO:0007829|PDB:4IC6"
SQ SEQUENCE 448 AA; 47493 MW; A986FC1387670AFF CRC64;
MQVIASFCSK PNENEFVGRR QLLSSVCSKI SQGDVVSHPP VSSVKVTQDW KSNLHELAVK
SVPSTTRRIL LTSLFMNLCF NPSRYLSALA LGDPSVATVE DVSPTVFPAG PLFPTEGRIV
QLFEKNTYSV VNIFDVTLRP QLKMTGVVEI PEGNGSGVVW DGQGYIVTNY HVIGNALSRN
PSPGDVVGRV NILASDGVQK NFEGKLVGAD RAKDLAVLKV DAPETLLKPI KVGQSNSLKV
GQQCLAIGNP FGFDHTLTVG VISGLNRDIF SQTGVTIGGG IQTDAAINPG NSGGPLLDSK
GNLIGINTAI FTQTGTSAGV GFAIPSSTVL KIVPQLIQFS KVLRAGINIE LAPDPVANQL
NVRNGALVLQ VPGKSLAEKA GLHPTSRGFA GNIVLGDIIV AVDDKPVKNK AELMKILDEY
SVGDKVTLKI KRGNEDLELK ISLEEKSS
