ID   DEH1_MORSB              Reviewed;         294 AA.
AC   Q01398;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Haloacetate dehalogenase H-1;
DE            EC=3.8.1.3;
GN   Name=dehH1;
OS   Moraxella sp. (strain B).
OG   Plasmid POU1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Moraxella; unclassified Moraxella.
OX   NCBI_TaxID=118147;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-10.
RX   PubMed=1512562; DOI=10.1099/00221287-138-7-1317;
RA   Kawasaki H., Tsuda K., Matsushita I., Tonomura K.;
RT   "Lack of homology between two haloacetate dehalogenase genes encoded on a
RT   plasmid from Moraxella sp. strain B.";
RL   J. Gen. Microbiol. 138:1317-1323(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a haloacetate + H2O = a halide anion + glycolate + H(+);
CC         Xref=Rhea:RHEA:11044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:29805, ChEBI:CHEBI:85638; EC=3.8.1.3;
CC   -!- MISCELLANEOUS: Two haloacetate dehalogenase enzymes exist in Moraxella
CC       strain B. DehH1 acts predominantly on fluoroacetate, dehH2 acts on
CC       chloro-, bromo- and iodoacetate, but not on fluoroacetate.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Epoxide hydrolase
CC       family. {ECO:0000305}.
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DR   EMBL; D90422; BAA14412.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q01398; -.
DR   SMR; Q01398; -.
DR   ESTHER; morsp-deh1; Haloacetate_dehalogenase.
DR   KEGG; ag:BAA14412; -.
DR   BioCyc; MetaCyc:MON-15937; -.
DR   GO; GO:0018785; F:haloacetate dehalogenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR000639; Epox_hydrolase-like.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   PRINTS; PR00412; EPOXHYDRLASE.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Plasmid.
FT   CHAIN           1..294
FT                   /note="Haloacetate dehalogenase H-1"
FT                   /id="PRO_0000207070"
FT   DOMAIN          27..277
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        105
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        272
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   294 AA;  33308 MW;  7BA7F07359182703 CRC64;
     MDFPGFKNST VTVDGVDIAY TVSGEGPPVL MLHGFPQNRA MWARVAPQLA EHHTVVCADL
     RGYGDSDKPK CLPDRSNYSF RTFAHDQLCV MRHLGFERFH LVGHDRGGRT GHRMALDHPE
     AVLSLTVMDI VPTYAMFMNT NRLVAASYWH WYFLQQPEPF PEHMIGQDPD FFYETCLFGW
     GATKVSDFDQ QMLNAYRESW RNPAMIHGSC SDYRAAATID LEHDSADIQR KVECPTLVFY
     GSKGQMGQLF DIPAEWAKRC NNTTNASLPG GHFFVDQFPA ETSEILLKFL ARNG