ID   DEH2_MORSB              Reviewed;         224 AA.
AC   Q01399;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Haloacetate dehalogenase H-2;
DE            EC=3.8.1.3;
GN   Name=dehH2;
OS   Moraxella sp. (strain B).
OG   Plasmid POU1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC   Moraxella; unclassified Moraxella.
OX   NCBI_TaxID=118147;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-10.
RX   PubMed=1512562; DOI=10.1099/00221287-138-7-1317;
RA   Kawasaki H., Tsuda K., Matsushita I., Tonomura K.;
RT   "Lack of homology between two haloacetate dehalogenase genes encoded on a
RT   plasmid from Moraxella sp. strain B.";
RL   J. Gen. Microbiol. 138:1317-1323(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a haloacetate + H2O = a halide anion + glycolate + H(+);
CC         Xref=Rhea:RHEA:11044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16042, ChEBI:CHEBI:29805, ChEBI:CHEBI:85638; EC=3.8.1.3;
CC   -!- MISCELLANEOUS: Two haloacetate dehalogenase enzymes exist in Moraxella
CC       strain B. DehH1 acts predominantly on fluoroacetate, dehH2 acts on
CC       chloro-, bromo- and iodoacetate, but not on fluoroacetate.
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. S-2-
CC       haloalkanoic acid dehalogenase family. {ECO:0000305}.
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DR   EMBL; D90423; BAA14413.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q01399; -.
DR   SMR; Q01399; -.
DR   GO; GO:0018785; F:haloacetate dehalogenase activity; IEA:UniProtKB-EC.
DR   CDD; cd02588; HAD_L2-DEX; 1.
DR   Gene3D; 1.10.150.240; -; 1.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR006328; 2-HAD.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR006439; HAD-SF_hydro_IA.
DR   InterPro; IPR041492; HAD_2.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR023198; PGP-like_dom2.
DR   Pfam; PF13419; HAD_2; 1.
DR   PRINTS; PR00413; HADHALOGNASE.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   TIGRFAMs; TIGR01493; HAD-SF-IA-v2; 1.
DR   TIGRFAMs; TIGR01509; HAD-SF-IA-v3; 1.
DR   TIGRFAMs; TIGR01428; HAD_type_II; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Plasmid.
FT   CHAIN           1..224
FT                   /note="Haloacetate dehalogenase H-2"
FT                   /id="PRO_0000079167"
FT   ACT_SITE        10
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   224 AA;  25346 MW;  CE80D0272DF89495 CRC64;
     MKKIEAIAFD MYGTLYDVHS VVDACEKQYP GKGKDISVLW RQKQLEYAWL RCLMGQYIKF
     EEATANALTY TCNQMKLDCD EGSAMRLTEE YLRLKPFPEV RGALRALRQR GMRLAILSNG
     STETIHDVVH NSGVEGEFEH LISVDSARAY KPHPLAYELG EEAFGISRES ILFVSSNPWD
     VSGAKAFGYQ VCWINRYGFA FDELGQTPDF TVPVMDAIVH LIAV