DEJP_DROME
ID DEJP_DROME Reviewed; 54 AA.
AC P81160; B3DMS6;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Ductus ejaculatorius peptide 99B;
DE Flags: Precursor;
GN Name=Dup99B; ORFNames=CG33495;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-37.
RC STRAIN=Berkeley;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-45, PROTEIN SEQUENCE OF 30-52,
RP PYROGLUTAMATE FORMATION AT GLN-22, GLYCOSYLATION, DISULFIDE BONDS,
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=Oregon-R; TISSUE=Ductus ejaculatorius;
RX PubMed=11846801; DOI=10.1046/j.0014-2956.2001.02733.x;
RA Saudan P., Hauck K., Soller M., Choffat Y., Ottiger M., Sporri M., Ding Z.,
RA Hess D., Gehrig P.M., Klauser S., Hunziker P., Kubli E.;
RT "Ductus ejaculatorius peptide 99B (DUP99B), a novel Drosophila melanogaster
RT sex-peptide pheromone.";
RL Eur. J. Biochem. 269:989-997(2002).
CC -!- FUNCTION: Induces post-mating responses; increased oviposition and
CC reduced receptivity. {ECO:0000269|PubMed:11846801}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11846801}.
CC -!- TISSUE SPECIFICITY: Ductus ejaculatorius.
CC {ECO:0000269|PubMed:11846801}.
CC -!- SIMILARITY: To paragonial peptide B. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACD81728.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=ACD81738.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=ACD81752.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=ACD81760.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAS65225.1; -; Genomic_DNA.
DR EMBL; BT032714; ACD81728.1; ALT_INIT; mRNA.
DR EMBL; BT032724; ACD81738.1; ALT_INIT; mRNA.
DR EMBL; BT032738; ACD81752.1; ALT_INIT; mRNA.
DR EMBL; BT032746; ACD81760.1; ALT_INIT; mRNA.
DR RefSeq; NP_996305.1; NM_206582.2.
DR AlphaFoldDB; P81160; -.
DR STRING; 7227.FBpp0088975; -.
DR GlyGen; P81160; 1 site.
DR iPTMnet; P81160; -.
DR PaxDb; P81160; -.
DR PRIDE; P81160; -.
DR DNASU; 2768691; -.
DR EnsemblMetazoa; FBtr0089608; FBpp0088975; FBgn0250832.
DR GeneID; 2768691; -.
DR KEGG; dme:Dmel_CG33495; -.
DR CTD; 2768691; -.
DR FlyBase; FBgn0250832; Dup99B.
DR VEuPathDB; VectorBase:FBgn0250832; -.
DR GeneTree; ENSGT00940000176695; -.
DR HOGENOM; CLU_213488_0_0_1; -.
DR OMA; REKWCRL; -.
DR PhylomeDB; P81160; -.
DR BioGRID-ORCS; 2768691; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 2768691; -.
DR PRO; PR:P81160; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0250832; Expressed in male reproductive gland and 11 other tissues.
DR ExpressionAtlas; P81160; baseline and differential.
DR Genevisible; P81160; DM.
DR GO; GO:0005615; C:extracellular space; HDA:FlyBase.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IPI:FlyBase.
DR GO; GO:0005179; F:hormone activity; IEA:InterPro.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:FlyBase.
DR GO; GO:0007621; P:negative regulation of female receptivity; TAS:FlyBase.
DR GO; GO:0045434; P:negative regulation of female receptivity, post-mating; IMP:FlyBase.
DR GO; GO:0018991; P:oviposition; IMP:FlyBase.
DR GO; GO:0046662; P:regulation of oviposition; TAS:FlyBase.
DR GO; GO:0019953; P:sexual reproduction; HEP:FlyBase.
DR InterPro; IPR012608; Sex_peptide.
DR Pfam; PF08138; Sex_peptide; 1.
PE 1: Evidence at protein level;
KW Behavior; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000269|PubMed:11846801"
FT PEPTIDE 22..52
FT /note="Ductus ejaculatorius peptide 99B"
FT /id="PRO_0000021103"
FT PROPEP 53..54
FT /id="PRO_0000021104"
FT MOD_RES 22
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:11846801"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:11846801"
FT DISULFID 40..52
FT /evidence="ECO:0000269|PubMed:11846801"
SQ SEQUENCE 54 AA; 6245 MW; 7D764665A2D5EFB5 CRC64;
MKTPLFLLLV VLASLLGLAL SQDRNDTEWI QSQKDREKWC RLNLGPYLGG RCRK
